Network Pharmacology-based Investigation of the Underlying Mechanism of Panax notoginseng Treatment of Diabetic Retinopathy
| Autor: | Xiaolin Tong, Chunli Piao, Han Wang, Fengmei Lian, Zheyu Sun, Naiwen Zhang, Xuemin Wu, De Jin |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Models
Molecular Chinese patent medicine Databases Factual Angiogenesis Molecular Conformation Panax notoginseng Traditional Chinese medicine Pharmacology 03 medical and health sciences 0302 clinical medicine Drug Discovery medicine Humans network pharmacology Medicine Chinese Traditional 030304 developmental biology 0303 health sciences Diabetic Retinopathy biology treatment Mechanism (biology) business.industry Organic Chemistry Combinatorial Chemistry & High Throughput Screening vascular disease General Medicine Diabetic retinopathy medicine.disease biology.organism_classification Computer Science Applications Vascular endothelial growth factor A 030220 oncology & carcinogenesis molecular mechanism business Systems pharmacology Drugs Chinese Herbal |
| Zdroj: | Combinatorial Chemistry & High Throughput Screening |
| ISSN: | 1875-5402 |
| Popis: | Background: Panax notoginseng, a Chinese herbal medicine, has been widely used to treat vascular diseases. Diabetic retinopathy (DR) is one of the complications of diabetic microangiopathy. According to recent studies, the application of Panax notoginseng extract and related Chinese patent medicine preparations can significantly improve DR. However, the pharmacological mechanisms remain unclear. Therefore, the purpose of this study was to decipher the potential mechanism of Panax notoginseng treatment of DR using network pharmacology. Methods: We evaluated and screened the active compounds of Panax notoginseng using the Traditional Chinese Medicine Systems Pharmacology database and collected potential targets of the compounds by target fishing. A multi-source database was also used to organize targets of DR. The potential targets as the treatment of DR with Panax notoginseng were then obtained by matching the compound targets with the DR targets. Using protein-protein interaction networks and topological analysis, interactions between potential targets were identified. In addition, we also performed gene ontology-biological process and pathway enrichment analysis for the potential targets by using the Biological Information Annotation Database. Results: Eight active ingredients of Panax notoginseng and 31 potential targets for the treatment of DR were identified. The screening and enrichment analysis revealed that the treatment of DR using Panax notoginseng primarily involved 28 biological processes and 10 related pathways. Further analyses indicated that angiogenesis, inflammatory reactions, and apoptosis may be the main processes involved in the treatment of DR with Panax notoginseng. In addition, we determined that the mechanism of intervention of Panax notoginseng in treating DR may involve five core targets, VEGFA, MMP-9, MMP-2, FGF2, and COX-2. Conclusion: Panax notoginseng may treat diabetic retinopathy through the mechanism of network pharmacological analysis. The underlying molecular mechanisms were closely related to the intervention of angiogenesis, inflammation, and apoptosis with VEGFA, MMP-9, MMP-2, FGF2, and COX-2 being possible targets. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|