Comparison of the frequencies of arginines in heavy chain CDR3 of antibodies expressed in the primary B-cell repertoires of autoimmune-prone and normal mice
| Autor: | Meera R. Krishnan, Tony N. Marion |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Male
Arginine Immunology Molecular Sequence Data Antibody Affinity Immunoglobulin Variable Region DNA Single-Stranded chemical and pharmacologic phenomena Autoimmune Diseases Pathogenesis chemistry.chemical_compound Mice medicine Animals B cell B-Lymphocytes Mice Inbred BALB C biology Base Sequence Mice Inbred NZB Repertoire Antibodies Monoclonal General Medicine DNA Molecular biology Chromatin medicine.anatomical_structure chemistry Antibody Formation biology.protein Immunoglobulin heavy chain Female Immunoglobulin Light Chains Antibody Immunoglobulin Heavy Chains Recombination |
| Zdroj: | Scandinavian journal of immunology. 48(3) |
| ISSN: | 0300-9475 |
| Popis: | Because the pathogenesis of anti-DNA Ab in SLE is correlated to Ab specificity for native DNA (dsDNA), understanding how such specificity is generated is important. The VH structures of most autoimmune anti-DNA antibodies include at least one arginine in VH–CDR3; moreover, antibody specificity for dsDNA can be correlated to the relative position of arginines in VH–CDR3. The coding sequences for most VH–CDR3 arginines among the anti-DNA MoAbs we have studied to date appeared to have been encoded by sequences generated during V–D–J recombination and would have been expressed in the primary B-cell repertoire. The frequency at which arginine codons are generated during V–D–J recombination therefore could potentially influence the frequency at which DNA-specific B cells are generated in the primary B-cell repertoire. The present study was undertaken to determine whether a higher percentage of B cells in the primary, preautoimmune repertoire of autoimmune-prone (NZB × NZW)F1 mice have immunoglobulin heavy chains with at least one VH–CDR3 arginine compared to B cells in the primary, preimmune repertoire of non-autoimmune-prone BALB/c mice. The present results indicate that mature B cells in preautoimmune (NZB × NZW)F1 mice, whether specific for DNA or not, are no more likely to have heavy chains with VH–CDR3 arginines than are B cells in BALB/c mice. The high frequency of recurrence of VH–CDR3 arginines among autoimmune anti-DNA in (NZB × NZW)F1 mice would appear to derive from the selective oligoclonal expansion of selected B cells that express such structures. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text