Adoptive Transfer of Diabetes in BB Rats Induced by CD4 T Lymphocytes
| Autor: | Dominique Duhamel, Marie-Dominique Métroz-Dayer, Christine Brideau, Philippe Poussier, Andrew Mouland |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
CD4-Positive T-Lymphocytes
Male Interleukin 2 medicine.medical_specialty Adoptive cell transfer Cellular immunity Receptors Antigen T-Cell alpha-beta Endocrinology Diabetes and Metabolism Lymphocyte Receptors Antigen T-Cell Stimulation Cell Separation Lymphocyte Activation Diabetes Mellitus Experimental Injections chemistry.chemical_compound Internal medicine Concanavalin A Internal Medicine medicine Splenocyte Animals Rats Inbred BB Cells Cultured biology business.industry Ionomycin Immunization Passive Rats Inbred Strains DNA T-Lymphocytes Helper-Inducer Rats Endocrinology medicine.anatomical_structure chemistry biology.protein Tetradecanoylphorbol Acetate Female business Cell Division Spleen medicine.drug |
| Zdroj: | Diabetes. 39:928-932 |
| ISSN: | 1939-327X 0012-1797 |
| DOI: | 10.2337/diab.39.8.928 |
| Popis: | Unseparated splenocytes (SPCs) or purified SPC subsets from diabetes-prone BB (BBdp) or diabetic BB (BBd) rats were activated in vitro with either phorbol myristate acetate (PMA) and ionomycin (I) or concanavalin A (ConA). Such activated SPCs were then injected intravenously into 30-day-old BBdp rats, and their capacity to induce adoptive transfer (AT) of diabetes was studied. The proliferative response in vitro of BBd unseparated SPCs or purified W3/13+ SPCs (i.e., T lymphocytes + large granular lymphocytes) to PMA + I far exceeded that of ConA, resulting in mean stimulation indices of 68 and 112 (PMA + I) and 1.9 and 30 (ConA). The incidence of AT was similar when equal numbers of unseparated SPCs from the same BBd donor were injected after activation by either PMA + I + interleukin 2 (Pll) or ConA (57 vs. 50%, respectively); however, injection of Pll-activated and macrophage-depleted W3/13+ SPCs from BBd animals resulted in a significantly higher incidence of AT (90%, P < 0.05). As few as 0.5 × 106 Pll-activated W3/13+ SPCs were sufficient to induce AT. Sixteen percent of recipients developed diabetes after injection of activated W3/13+ cells from 40-dayold BBdp donors. To determine which W3/13+ cells might mediate such transfer, purified and Pll-preactivated CD4 T lymphocytes from BBd rats were injected, and they succeeded in AT in 44% of the recipients. Preactivated BBd B lymphocytes were unable to induce AT. Although a possible role for large granular lymphocytes cannot be excluded, the results demonstrate that in the BB rat, the β-cell destruction can be induced by CD4 T lymphocytes. |
| Databáze: | OpenAIRE |
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