The cortical stab injury induces beading of fibers expressing ecto-nucleoside triphosphate diphosphohydrolase 3
| Autor: | Mirjana Stojiljkovic, Danijela Stojkov, Irena Lavrnja, Sanja Pekovic, Ivana Bjelobaba, Ana Parabucki, Nadežda Nedeljković |
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| Rok vydání: | 2010 |
| Předmět: |
Male
Protein subunit Punctures Biology Nerve Fibers Myelinated Gene Expression Regulation Enzymologic 5'-nucleotidase Stereotaxic Techniques Lesion 03 medical and health sciences chemistry.chemical_compound Adenosine Triphosphate 0302 clinical medicine Microtubule Cortex (anatomy) medicine Extracellular Animals Pyrophosphatases Rats Wistar 030304 developmental biology Cerebral Cortex 0303 health sciences General Neuroscience Molecular biology Rats Cell biology medicine.anatomical_structure chemistry Brain Injuries Nucleoside triphosphate medicine.symptom Adenosine triphosphate 030217 neurology & neurosurgery |
| Zdroj: | Neuroscience |
| ISSN: | 0306-4522 |
| DOI: | 10.1016/j.neuroscience.2010.06.063 |
| Popis: | The ecto-nucleoside triphosphate diphosphohydrolase 3 (NTPDase3), an enzyme involved in degradation of extracellular adenosine triphosphate (ATP), is expressed on nerve fibers in different brain regions, including cortex. Here we studied the expression and role of this enzyme after unilateral cortical stab injury in rats. In cortical sections of control rats, NTPDase3 immunoreactivity was associated with two types of fibers: thin processes, occasionally with small mushroom-like protrusions and slightly thicker fibers with more pronounced and more frequent varicosities, whereas immunopositive neuronal perycaria were never observed. Although NTPDase3-positive thin processes and thicker fibers, by general appearance, size and shape, could be dendrites and axons, respectively, they were never immunopositive for microtubule associated protein-2 or neurofilament H subunit. Cortical stab injury induced rapid (within 4 hours) focal varicose swelling that evolved over time to prominent beading of NTPDase3-positive fibers. The NTPDase3-positive fibers in all experimental groups also abundantly express NTPDase1, ecto-5'-nucleotidase and P2X2 receptor channels. Because the brain injury causes a massive ATP release, it is reasonable to conclude that purinoreceptors and ectonucleotidases play an important role in the process of neuritic beading. (C) 2010 IBRO. Published by Elsevier Ltd. All rights reserved. Serbian Ministry of Science and Technology [143005] |
| Databáze: | OpenAIRE |
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