Anxiolytic properties of green tea polyphenol (-)-epigallocatechin gallate (EGCG)
| Autor: | Karen Thethi, Janique Guiramand, Max Récasens, Fabien Lanté, Tangui Maurice, Magali Nedjar, Michel Vignes |
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| Přispěvatelé: | Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM), Plasticité cérébrale (PC), Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
Male
Patch-Clamp Techniques MESH: Hippocampus MESH: Drug Interactions MESH: Neurons Convulsants MESH: gamma-Aminobutyric Acid Pharmacology Epigallocatechin gallate Anxiety Hippocampus Catechin Membrane Potentials MESH: Dose-Response Relationship Drug chemistry.chemical_compound Mice 0302 clinical medicine MESH: Convulsants MESH: Behavior Animal Drug Interactions MESH: Animals MESH: Avoidance Learning Cells Cultured gamma-Aminobutyric Acid Neurons 0303 health sciences Electroshock Behavior Animal GABAA receptor Chemistry General Neuroscience food and beverages MESH: Electric Stimulation 3. Good health Biochemistry MESH: Chlordiazepoxide [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] medicine.drug MESH: Cells Cultured MESH: Electroshock medicine.drug_class MESH: Catechin Neurotransmission Inhibitory postsynaptic potential Anxiolytic complex mixtures gamma-Aminobutyric acid Chlordiazepoxide 03 medical and health sciences MESH: Patch-Clamp Techniques medicine Avoidance Learning Reaction Time Animals MESH: Membrane Potentials Maze Learning Molecular Biology MESH: Mice 030304 developmental biology Benzodiazepine Dose-Response Relationship Drug MESH: Anxiety MESH: Maze Learning MESH: Embryo Mammalian Embryo Mammalian Electric Stimulation MESH: Male MESH: Reaction Time Disease Models Animal MESH: Anti-Anxiety Agents Anti-Anxiety Agents MESH: Carbolines Neurology (clinical) MESH: Disease Models Animal 030217 neurology & neurosurgery Developmental Biology Carbolines |
| Zdroj: | Brain Research Brain Research, Elsevier, 2006, 1110 (1), pp.102-15. ⟨10.1016/j.brainres.2006.06.062⟩ |
| ISSN: | 0006-8993 |
| DOI: | 10.1016/j.brainres.2006.06.062⟩ |
| Popis: | International audience; Naturally occurring polyphenols are potent antioxidants. Some of these compounds are also ligands for the GABA(A) receptor benzodiazepine site. This feature endows them with sedative properties. Here, the anxiolytic activity of the green tea polyphenol (-)-epigallocatechin gallate (EGCG) was investigated after acute administration in mice, using behavioral tests (elevated plus-maze and passive avoidance tests) and by electrophysiology on cultured hippocampal neurons. Patch-clamp experiments revealed that EGCG (1-10 muM) had no effect on GABA currents. However, EGCG reversed GABA(A) receptor negative modulator methyl beta-carboline-3-carboxylate (beta-CCM) inhibition on GABA currents in a concentration dependent manner. This was also observed at the level of synaptic GABA(A) receptors by recording spontaneous inhibitory synaptic transmission. In addition, EGCG consistently inhibited spontaneous excitatory synaptic transmission. Behavioral tests indicated that EGCG exerted both anxiolytic and amnesic effects just like the benzodiazepine drug, chlordiazepoxide. Indeed, EGCG in a dose-dependent manner both increased the time spent in open arms of the plus-maze and decreased the step-down latency in the passive avoidance test. GABA(A) negative modulator beta-CCM antagonized EGCG-induced amnesia. Finally, state-dependent learning was observable after chlordiazepoxide and EGCG administration using a modified passive avoidance procedure. Optimal retention was observed only when animals were trained and tested in the same state (veh-veh or drug-drug) and significant retrieval alteration was observed in different states (veh-drug or drug-veh). Moreover, EGCG and chlordiazepoxide fully generalized in substitution studies, indicating that they induced indistinguishable chemical states for the brain. Therefore, our data support that EGCG can induce anxiolytic activity which could result from an interaction with GABA(A) receptors. |
| Databáze: | OpenAIRE |
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