Diacylglycerol kinases activate tobacco NADPH oxidase-dependent oxidative burst in response to cryptogein

Autor: Jérôme Fromentin, Sébastien Mongrand, Jean-Luc Cacas, Emmanuelle Jeannette, Dominique Thomas, Patricia Gerbeau-Pissot, Françoise Simon-Plas, Tetiana Kalachova, Eric Ruelland, Catherine Cantrel
Přispěvatelé: Agroécologie [Dijon], Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Laboratoire de Physiologie Cellulaire et Moléculaire des Plantes (UR5), Université Pierre et Marie Curie - Paris 6 (UPMC), Institut d'écologie et des sciences de l'environnement de Paris (iEES), Centre National de la Recherche Scientifique (CNRS)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Recherche Agronomique (INRA), Laboratoire de biogenèse membranaire (LBM), Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Centre National de la Recherche Scientifique (CNRS), Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Laboratoire de Physiologie Cellulaire et Moléculaire des Plantes ( UR5 ), Université Pierre et Marie Curie - Paris 6 ( UPMC ), Institut d'écologie et des sciences de l'environnement de Paris ( IEES ), Institut National de la Recherche Agronomique ( INRA ) -Université Pierre et Marie Curie - Paris 6 ( UPMC ) -Université Paris-Est Créteil Val-de-Marne - Paris 12 ( UPEC UP12 ) -Centre National de la Recherche Scientifique ( CNRS ), Laboratoire de biogenèse membranaire ( LBM ), Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique ( CNRS ), Institut d'écologie et des sciences de l'environnement de Paris (IEES)
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0106 biological sciences
0301 basic medicine
Physiology
diacylglycerol kinase
Plant Science
01 natural sciences
cryptogein
chemistry.chemical_compound
Cluster Analysis
phospholipase C
Phylogeny
Plant Proteins
Respiratory Burst
chemistry.chemical_classification
reactive oxygen species
NADPH oxidase
biology
Kinase
[ SDV.BV.PEP ] Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy
Phosphatidic acid
Plants
Genetically Modified
Respiratory burst
phosphatidic acid
Biochemistry
Gain of Function Mutation
Phosphatidic Acids
Cell Line
Fungal Proteins
03 medical and health sciences
Tobacco
Gene Silencing
Protein Kinase Inhibitors
Diacylglycerol kinase
[ SDE.BE ] Environmental Sciences/Biodiversity and Ecology
Reactive oxygen species
RBOHD
Phospholipase C
Pathogen-Associated Molecular Pattern Molecules
NADPH Oxidases
[SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy
Enzyme Activation
MicroRNAs
030104 developmental biology
Enzyme
chemistry
Type C Phospholipases
biology.protein
[SDE.BE]Environmental Sciences/Biodiversity and Ecology
010606 plant biology & botany
microbial-associated molecular pattern
Zdroj: Plant, Cell and Environment
Plant, Cell and Environment, Wiley, 2016, 40 (4), pp.585-598. ⟨10.1111/pce.12771⟩
Plant, Cell and Environment, 2016, 40 (4), pp.585-598. ⟨10.1111/pce.12771⟩
Plant, Cell and Environment, Wiley, 2016, 〈10.1111/pce.12771〉
Plant, Cell and Environment, Wiley, 2016, ⟨10.1111/pce.12771⟩
ISSN: 0140-7791
1365-3040
Popis: SPE IPM UB INRA SUPDAT; International audience; Cryptogein is a 10 kDa-protein secreted by the oomycete Phytophthora cryptogea that activates defence mechanisms in tobacco plants. Among early signalling events triggered by this microbial-associated molecular pattern is a transient apoplastic oxidative burst which is dependent on the NADPH oxidase activity of the RESPIRATORY BURST OXIDASE HOMOLOG (RBOH) isoform D. Using radioactive [33P]-orthophosphate labelling of tobacco Bright Yellow-2 suspension cells, we here provide in vivo evidence for a rapid accumulation of phosphatidic acid (PA) in response to cryptogein due to the coordinated onset of phosphoinositide-dependent phospholipase C and diacylglycerol kinase (DGK) activities. Both enzyme specific inhibitors and silencing of the phylogenetic cluster III of the tobacco DGK family were found to reduce PA production upon elicitation and to strongly decrease the RBOHD-mediated oxidative burst. Therefore, it appears that PA originating from DGK controls NADPH-oxidase activity. Amongst cluster III DGKs, the expression of DGK5-like was up-regulated in response to cryptogein. Besides DGK5-like is likely to be the main cluster III DGK isoform silenced in one of our mutant line, making it a strong candidate for the observed response to cryptogein. The relevance of these results is discussed with regard to early signalling lipid-mediated events in plant immunity.
Databáze: OpenAIRE
Externí odkaz: