WITHAFERIN A INDUCES APOPTOSIS IN RAT C6 GLIOMA CELLS THROUGH REGULATING NF- ΚB NUCLEAR TRANSLOCATION AND ACTIVATION OF CASPASE CASCADE
| Autor: | Weichen Hou, Xiaoxue Bai, Qun Liu, Xiao-hui Miao, Lei Song, Lianjun Ma |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
anti-proliferative
0301 basic medicine Withaferin A C6 glioma cells anti-proliferative anti-inflammatory caspase apoptosis medicine.drug_class caspase Apoptosis DNA Fragmentation Withania Pharmacology Article Anti-inflammatory 03 medical and health sciences chemistry.chemical_compound Downregulation and upregulation Cell Line Tumor Drug Discovery medicine Animals Cytotoxicity Withanolides Caspase anti-inflammatory Cell Proliferation bcl-2-Associated X Protein Inflammation biology Plant Extracts NF-kappa B Biological Transport NF-κB Glioma Antineoplastic Agents Phytogenic Rats Enzyme Activation 030104 developmental biology Proto-Oncogene Proteins c-bcl-2 Complementary and alternative medicine chemistry Withaferin A C6 glioma cells Caspases biology.protein DNA fragmentation Phytotherapy |
| Zdroj: | African Journal of Traditional, Complementary, and Alternative Medicines African Journal of Traditional, Complementary and Alternative Medicines; Vol 14, No 2 (2017); 319-324 |
| ISSN: | 2505-0044 0189-6016 |
| Popis: | Background: The demand for the chemopreventive drug from the plant source is increasing in recent times, owing to its various biological activities without any adverse effect. The intention of this current study was to examine the antiglioma effect of Withaferin A (WFA) on C6 glioma cell line model.Materials and Methods: C6 glioma cells were administrated with different concentration of WFA (50, 100, 200 and 500 μg/mL) and DMSO (control) group to examine its anti-proliferative, anti-inflammatory and pro-apoptotic activities.Results: Treatment with WFA showed a significant decline in the glioma cell count in a dose-dependent manner and thus proving its anti-proliferative effect. Similarly, inflammatory markers were also substantially lowered upon treatment with different concentration of WFA. However, DNA fragmentation and apoptotic markers like Caspase-3 and 9 were concomitantly enhanced after co-cultured with different concentration of WFA and thus exhibiting its cytotoxicity efficacy. Furthermore, the protein expression of Bcl2 and Bax were markedly downregulated and upregulated respectively; upon treatment with WFA on C6 glioma cells.Conclusion: The outcome of this study evidently demonstrates that C6 glioma cells co-cultured with increased concentration of WFA, showed an anti-proliferative, anti-inflammatory and pro-apoptotic effect in a dose-dependent fashion.Keywords: Withaferin A, C6 glioma cells, anti-proliferative, anti-inflammatory, caspase, apoptosis |
| Databáze: | OpenAIRE |
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