Transposon accumulation lines uncover histone H2A.Z-driven integration bias towards environmentally responsive genes
| Autor: | Jean-Marc Aury, Colot, Erwann Caillieux, Mathilde Etcheverry, Leandro Quadrana, Baillet, Julie Guy, Wincke P, Stefan Engelen, Arthur Gilly, Silveira Ab, Mohammed-Amine Madoui |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
Transposable element Genetics 0303 health sciences education.field_of_study Genome evolution biology Population Genomics 01 natural sciences Chromatin 03 medical and health sciences Histone biology.protein Epigenetics education Gene 030304 developmental biology 010606 plant biology & botany |
| DOI: | 10.1101/447870 |
| Popis: | Inherited transposition events are important drivers of genome evolution but because transposable element (TE) mobilization is usually rare, its impact on the creation of genetic variation remains poorly characterized. Here, we used a population of A. thaliana epigenetic recombinant inbred lines (epiRILs) to characterize >8000 de novo insertions produced by several TEs families also active in nature. Integration was strongly biased towards genes, with evident deleterious effects. Biases were TE family-specific and associated with distinct chromatin features. Notably, we demonstrate that the histone variant H2A.Z guides the preferential integration of Ty1/Copia LTR-retrotransposons within environmentally responsive genes and that this guiding function is evolutionary conserved. Finally, we uncover an important role for epigenetic silencing in exacerbating or alleviating the effects of TE insertions on target genes. These findings establish chromatin as a major determinant of the spectrum and functional impact of TE-generated mutations, with important implications for adaptation and evolution. |
| Databáze: | OpenAIRE |
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