Intracellular Localization of Mycoplasma bovis in the Bronchiolar Epithelium of Experimentally Infected Calves
| Autor: | H. Tomioka, K. Uetsuka, T. Omori, T. Suzuki, T. Nunoya, F. Umeda |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Mycoplasma bovis
Pathology medicine.medical_specialty 040301 veterinary sciences Cattle Diseases Biology 030308 mycology & parasitology Pathology and Forensic Medicine law.invention 0403 veterinary science 03 medical and health sciences Antigen law Pneumonia Mycoplasma medicine Animals Bronchioles 0303 health sciences Lung General Veterinary 04 agricultural and veterinary sciences respiratory system Hyperplasia medicine.disease Epithelium respiratory tract diseases Lymphatic system medicine.anatomical_structure Cytoplasm Immunohistochemistry Cattle Electron microscope |
| Zdroj: | Journal of Comparative Pathology. 176:14-18 |
| ISSN: | 0021-9975 |
| DOI: | 10.1016/j.jcpa.2020.01.005 |
| Popis: | Lung tissues from calves infected experimentally with Mycoplasma bovis were examined by immunohistochemistry and electron microscopy. All inoculated calves had dark red areas of consolidation affecting both left and right lungs, which were characterized microscopically by subacute purulent bronchiolitis with hyperplasia of the surrounding lymphoid tissue. Immunohistochemically, M. bovis antigen was detected on the surface and inside the cytoplasm of bronchiolar epithelial cells in the pneumonic foci. The antigen was also found in the cytoplasm of phagocytes at the margin of bronchiolar exudates. Electron microscopically, numerous organisms were demonstrated in the immunohistochemically-positive sites. These findings suggest that M. bovis organisms adhere to the bronchiolar epithelium and at least some of them invade the epithelium. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect