Rho1- and Pkc1-dependent phosphorylation of the F-BAR protein Syp1 contributes to septin ring assembly
| Autor: | Franck Vandermoere, Alessio Bolognesi, Simonetta Piatti, Roberta Pascolutti, Yves Barral, Martial Séveno, Thibault Courtellemont, Maria Angeles Juanes, Laura Merlini |
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| Přispěvatelé: | Fédération de Mathématiques de l'Ecole Centrale Paris (FR3487), Ecole Centrale Paris-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS), Centre de recherche en Biologie Cellulaire (CRBM), Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Institute of Biochemistry, Eidgenössische Technische Hochschule - Swiss Federal Institute of Technology [Zürich] (ETH Zürich), Institut de Génomique Fonctionnelle - Montpellier GenomiX (IGF MGX), Institut de Génomique Fonctionnelle (IGF), Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS), BioCampus Montpellier (BCM), Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
rho GTP-Binding Proteins
Saccharomyces cerevisiae Proteins Saccharomyces cerevisiae GTPase macromolecular substances Biology Septin Gene Expression Regulation Fungal Cleavage furrow [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Phosphorylation Molecular Biology Cytoskeleton Protein Kinase C Protein kinase C Cytokinesis fungi Articles Cell Biology Cell biology Septin ring Septin ring assembly biological phenomena cell phenomena and immunity Carrier Proteins Septins Signal Transduction |
| Zdroj: | Molecular Biology of the Cell Molecular Biology of the Cell, American Society for Cell Biology, 2015, 26 (18), pp.3245-3262. ⟨10.1091/mbc.e15-06-0366⟩ Molecular Biology of the Cell, 26 (18) |
| ISSN: | 1939-4586 1059-1524 |
| Popis: | In many cell types, septins assemble into filaments and rings at the neck of cellular appendages and/or at the cleavage furrow to help compartmentalize the plasma membrane and support cytokinesis. How septin ring assembly is coordinated with membrane remodeling and controlled by mechanical stress at these sites is unclear. Through a genetic screen, we uncovered an unanticipated link between the conserved Rho1 GTPase and its effector protein kinase C (Pkc1) with septin ring stability in yeast. Both Rho1 and Pkc1 stabilize the septin ring, at least partly through phosphorylation of the membrane-associated F-BAR protein Syp1, which colocalizes asymmetrically with the septin ring at the bud neck. Syp1 is displaced from the bud neck upon Pkc1-dependent phosphorylation at two serines, thereby affecting the rigidity of the new-forming septin ring. We propose that Rho1 and Pkc1 coordinate septin ring assembly with membrane and cell wall remodeling partly by controlling Syp1 residence at the bud neck. Molecular Biology of the Cell, 26 (18) ISSN:1939-4586 ISSN:1059-1524 |
| Databáze: | OpenAIRE |
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