Validation of an ELISA for detection of neutrophil gelatinase-associated lipocalin (NGAL) in equine serum
| Autor: | Emil Tvermose, Stine Jacobsen, Gaby Van Galen, Malene Laurberg, Lise C. Berg |
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| Rok vydání: | 2018 |
| Předmět: |
General Veterinary
040301 veterinary sciences business.industry Coefficient of variation 030232 urology & nephrology Reproducibility of Results Horse Enzyme-Linked Immunosorbent Assay 04 agricultural and veterinary sciences Serum concentration Lipocalin 0403 veterinary science Andrology Neutrophil gelatinase-associated lipocalin 03 medical and health sciences 0302 clinical medicine Elevated serum creatinine Lipocalin-2 Creatinine Kidney injury Animals Medicine Horse Diseases Horses business |
| Zdroj: | Veterinary Clinical Pathology. 47:603-607 |
| ISSN: | 0275-6382 |
| Popis: | Background Neutrophil gelatinase-associated lipocalin (NGAL) has been shown to be a useful marker of kidney injury in people and dogs, but has not been described in horses. Objectives The aim of the study was to validate a commercially available porcine-specific ELISA to measure serum concentrations of equine NGAL. Methods Intra- and interassay imprecisions were evaluated by multiple measurements on equine serum pools. Assay inaccuracy was determined by the linearity under dilution. Overlapping performance was assessed by measuring NGAL concentrations in horses with normal and elevated serum creatinine levels. Results Intra- and interassay imprecision (coefficient of variation) ranged from 5.35% to 28.39%. The ELISA showed no signs of inaccuracy. Overlapping performance was acceptable, as the assay was able to detect the expected differences of NGAL levels in horses with normal and elevated serum creatinine concentrations. Conclusions Equine serum NGAL concentrations could be quantified reliably using the porcine-specific ELISA. |
| Databáze: | OpenAIRE |
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