A phase 1 trial extension to assess immunologic efficacy and safety of prime-boost vaccination with VXM01, an oral T cell vaccine against VEGFR2, in patients with advanced pancreatic cancer
| Autor: | Christoph Springfeld, Heinz Lubenau, Lars Grenacher, Lilli Podola, Ruhan Koc, Nicolas Hohmann, Klaus M. Breiner, Markus W. Büchler, Friedrich H. Schmitz-Winnenthal, Gerd Mikus, Phillip Knebel, Mariana Bucur, Marco Springer, Philipp Beckhove, Anne-Valerie Keller, Walter E. Haefeli, Thomas Schmidt, Sébastien Wieckowski, Tobias Friedrich |
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| Rok vydání: | 2018 |
| Předmět: |
lcsh:Immunologic diseases. Allergy
0301 basic medicine pancreatic cancer Immunology Priming (immunology) immune-oncology lcsh:RC254-282 salmonella typhi 03 medical and health sciences 0302 clinical medicine Antigen Pancreatic cancer medicine Immunology and Allergy Original Research business.industry vegfr2 Immunogenicity lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens medicine.disease human cd8+ t cells Gemcitabine Vaccination 030104 developmental biology Oncology oral vaccination 030220 oncology & carcinogenesis Concomitant anti-angiogenesis lcsh:RC581-607 business T-cell vaccine medicine.drug |
| Zdroj: | OncoImmunology, Vol 7, Iss 4 (2018) |
| ISSN: | 2162-402X |
| Popis: | VXM01 is a first-in-kind orally applied tumor vaccine based on live attenuated Salmonella typhi carrying an expression plasmid encoding VEGFR2, an antigen expressed on tumor vasculature and a stable and accessible target for anti-angiogenic intervention. A recent randomized, placebo-controlled, phase I dose-escalation trial in advanced pancreatic cancer patients demonstrated safety, immunogenicity and transient, T-cell response-related anti-angiogenic activity of four priming vaccinations applied within one week. We here evaluated whether monthly boost vaccinations are safe and can sustain increased frequencies of vaccine-specific T cells. Patients with advanced pancreatic cancer were randomly assigned at a ratio of 2:1 to priming with VXM01 followed by up to six monthly boost vaccinations, or placebo treatment. Vaccinations were applied orally at two alternative doses of either 106 colony-forming units (CFU) or 107 CFU, and concomitant treatment with standard-of-care gemcitabine during the priming phase, and any treatment thereafter, was allowed in the study. Immunomonitoring involved interferon-gamma (IFNγ) ELIspot analysis with long overlapping peptides spanning the entire VEGFR2 sequence. A total of 26 patients were treated. Treatment-related adverse events preferentially associated with VXM01 were decreases in lymphocyte numbers in the blood, increased frequencies of neutrophils and diarrhea. Eight out of 16 patients who received at least one boosting vaccination responded with pronounced, i.e. at least 3-fold, increase in VEGFR2-specific T cell response over baseline levels. In the VXM01 vaccination group, VEGFR2-specific T cells peaked preferentially during the boosting phase with an average 4-fold increase over baseline levels. In conclusion, prime/boost vaccination with VXM01 was safe and immunogenic and increased vaccine specific T cell responses compared with placebo treatment. |
| Databáze: | OpenAIRE |
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