Hybrid Capture II-based human papillomavirus detection, a sensitive test to detect in routine high-grade cervical lesions: a preliminary study on 1518 women
| Autor: | I Putaud, Marie Masure, Christine Clavel, J. P. Bory, C Mangeonjean, R Gabriel, M Lorenzato, Philippe Birembaut, Christian Quereux |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1999 |
| Předmět: |
Adult
Cancer Research Pathology medicine.medical_specialty Adolescent cervical cancer Cervix Uteri Sensitivity and Specificity Lesion Cytology Carcinoma Medicine Humans Papillomaviridae Human papillomavirus human papillomavirus Aged Invasive carcinoma biology business.industry Hybrid capture cytological screening Regular Article Middle Aged medicine.disease biology.organism_classification Oncology DNA Viral Female medicine.symptom business Viral load |
| Zdroj: | British Journal of Cancer |
| ISSN: | 1532-1827 0007-0920 |
| Popis: | Hybrid Capture II (HC-II) is a commercial human papillomavirus (HPV) detection test designed to detect 18 HPV types divided into high-risk and low-risk groups. We have tested 1647 scrapes from 1518 unselected women attending routine cytological screening by this assay for the detection of histologically proven high-grade lesions. The reliability of this test was also evaluated on 117 fresh cone biopsy samples. HPV DNA has been detected in 400 scrapes (24.3%), 296 containing a high-risk HPV (18.0%). All the smears evocative of high-grade lesions were positive for high-risk HPV, and high-risk HPV were detected in all the 34 cases presenting a histologically proven high-grade lesion and in 68 (97.1%) of the 70 cone biopsy samples showing a high-grade lesion or an invasive carcinoma. Thus, the sensitivity was superior to the sensitivity of cytology (85.3%). Nevertheless, the quantitative approach provided by the HC-II assay for the assessment of the viral load could not clearly distinguish among cases with or without high-grade lesions. Thus this assay is recommended for the screening of high-grade lesions on a large scale, in association with classic cytology. © 1999 Cancer Research Campaign |
| Databáze: | OpenAIRE |
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