Chromatin Preparation and Chromatin Immuno-precipitation from Drosophila Embryos
| Autor: | Eva Löser, Daniel Latreille, Nicola Iovino |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
biology Embryo biology.organism_classification Deep sequencing DNA sequencing Cell biology Chromatin 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Histone biology.protein Drosophila (subgenus) Chromatin immunoprecipitation 030217 neurology & neurosurgery |
| Zdroj: | Methods in Molecular Biology Methods in Molecular Biology ISBN: 9781493963782 |
| Popis: | This protocol provides specific details on how to perform Chromatin immunoprecipitation (ChIP) from Drosophila embryos. ChIP allows the matching of proteins or histone modifications to specific genomic regions. Formaldehyde-cross-linked chromatin is isolated and antibodies against the target of interest are used to determine whether the target is associated with a specific DNA sequence. This can be performed in spatial and temporal manner and it can provide information about the genome-wide localization of a given protein or histone modification if coupled with deep sequencing technology (ChIP-Seq). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|