CBL/CAP Is Essential for Mitochondria Respiration Complex I Assembly and Bioenergetics Efficiency in Muscle Cells
| Autor: | Cho-Cho Aye, Dean E. Hammond, Sergio Rodriguez-Cuenca, Mary K. Doherty, Phillip D. Whitfield, Marie M. Phelan, Chenjing Yang, Rafael Perez-Perez, Xiaoxin Li, Angels Diaz-Ramos, Gopal Peddinti, Matej Oresic, Antonio Vidal-Puig, Antonio Zorzano, Cristina Ugalde, Silvia Mora |
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| Přispěvatelé: | Rodriguez-Cuenca, Sergio [0000-0001-9635-0504], Doherty, Mary K [0000-0002-3480-4088], Peddinti, Gopal [0000-0002-8767-968X], Ugalde, Cristina [0000-0002-9742-1877], Mora, Silvia [0000-0002-8473-1497], Apollo - University of Cambridge Repository |
| Rok vydání: | 2023 |
| Předmět: |
CBL
Insulin/metabolism Cell Respiration Proto-Oncogene Proteins c-cbl/metabolism Muscle/metabolism insulin signalling Catalysis Mitocondris Inorganic Chemistry Mice Muscle Cells/metabolism insulin resistance Mitochondria/metabolism Animals Insulin Proto-Oncogene Proteins c-cbl Physical and Theoretical Chemistry Molecular Biology Spectroscopy Muscle Cells Organic Chemistry glucose transport Insulin resistance General Medicine Mitochondria Muscle/metabolism SORBS1 Computer Science Applications Mitochondria Mitochondria Muscle Glucose Glucosa Resistència a la insulina Energy Metabolism |
| Zdroj: | International Journal of Molecular Sciences Volume 24 Issue 4 Pages: 3399 Aye, C C, Hammond, D E, Rodriguez-Cuenca, S, Doherty, M K, Whitfield, P D, Phelan, M M, Yang, C, Perez-Perez, R, Li, X, Diaz-Ramos, A, Peddinti, G, Oresic, M, Vidal-Puig, A, Zorzano, A, Ugalde, C & Mora, S 2023, ' CBL/CAP Is Essential for Mitochondria Respiration Complex I Assembly and Bioenergetics Efficiency in Muscle Cells ', International Journal of Molecular Sciences, vol. 24, no. 4, 3399 . https://doi.org/10.3390/ijms24043399 |
| DOI: | 10.3390/ijms24043399 |
| Popis: | Peer reviewed: True Funder: European Regional Development Fund Funder: Scottish Funding Council Funder: Highlands and Islands Enterprise CBL is rapidly phosphorylated upon insulin receptor activation. Mice whole body CBL depletion improved insulin sensitivity and glucose clearance; however, the precise mechanisms remain unknown. We depleted either CBL or its associated protein SORBS1/CAP independently in myocytes and assessed mitochondrial function and metabolism compared to control cells. CBL- and CAP-depleted cells showed increased mitochondrial mass with greater proton leak. Mitochondrial respiratory complex I activity and assembly into respirasomes were reduced. Proteome profiling revealed alterations in proteins involved in glycolysis and fatty acid degradation. Our findings demonstrate CBL/CAP pathway couples insulin signaling to efficient mitochondrial respiratory function and metabolism in muscle. |
| Databáze: | OpenAIRE |
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