Optimization and validation of a micellar electrokinetic chromatographic method for the analysis of florfenicol
| Autor: | W. Van den Bossche, S Hillaert |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Florfenicol
Thiamphenicol Chromatography Sodium Clinical Biochemistry Pharmaceutical Science chemistry.chemical_element Florfenicol amine Quantitative determination Analytical Chemistry Electrokinetic phenomena chemistry.chemical_compound chemistry Drug Discovery medicine Selectivity Spectroscopy medicine.drug Antibacterial agent Chromatography Micellar Electrokinetic Capillary |
| Zdroj: | Journal of pharmaceutical and biomedical analysis. 36(3) |
| ISSN: | 0731-7085 |
| Popis: | We have optimized a micellar electrokinetic capillary chromatographic method for the separation of florfenicol and florfenicol amine, its degradation product. The separation was carried out using a 50 mM sodium borate buffer (pH 9.0) containing 25 mM of sodium dodecyl sulphate. The method selectivity was proven by the simultaneous separation of florfenicol and two structural antibiotics, chloramphenicol and thiamphenicol. The same system can also be applied for the quantitative determination of these antibiotics. The method was then validated regarding linearity, precision and accuracy. |
| Databáze: | OpenAIRE |
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