Identification of Cellular Recognition Sequence of Epimorphin and Critical Role of Cell/Epimorphin Interaction in Lung Branching Morphogenesis
| Autor: | Shogo Koshida, Yohei Hirai |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Cell type
Mesoderm Molecular Sequence Data Cell Biophysics Plasma protein binding Biology Biochemistry Epithelium Cell membrane Mice Organ Culture Techniques Recognition sequence medicine Animals Amino Acid Sequence Lung Molecular Biology Peptide sequence Mice Inbred ICR Membrane Glycoproteins Cell Membrane Cell Biology Peptide Fragments Cell biology medicine.anatomical_structure Signal transduction Protein Binding Signal Transduction |
| Zdroj: | Biochemical and Biophysical Research Communications. 234:522-525 |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.1997.6673 |
| Popis: | A mesenchymal protein, epimorphin, is known to bind directly to the cell surface through its central portion and to act as a signaling molecule for epithelial morphogenesis. Utilizing several recombinant polypeptides and synthetic peptides, we identified the cellular recognition sequence of epimorphin in the central portion of this molecule (amino acids 105-123, NGNRTSVDLRIRRTQHSVL; termed NL-peptide sequence). Interestingly, although a model cell type bound to the NL-peptide as strong as to the full-length epimorphin, this peptide itself didn't induce the cellular functional responses so far tested. We found that the NL-peptide behaved as an antagonist for the endogenous epimorphin and severely perturbed lung branching morphogenesis in organ culture. These results not only revealed a part of the functional mechanism of epimorphin but also demonstrated that cell/ epimorphin interaction through the NL-peptide sequence is a critical step for lung epithelial morphogenesis. |
| Databáze: | OpenAIRE |
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