| Autor: |
Sandra Verstraelen, Gareth Maglennon, Karen Hollanders, Francis Boonen, Els Adriaens, Nathalie Alépée, Agnieszka Drzewiecka, Katarzyna Gruszka, Helena Kandarova, Jamin A. Willoughby, Robert Guest, Jane Schofield, An R. Van Rompay |
| Rok vydání: |
2017 |
| Předmět: |
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| Zdroj: |
Toxicology in vitro : an international journal published in association with BIBRA. 49 |
| ISSN: |
1879-3177 |
| Popis: |
Assessment of ocular irritation potential is an international regulatory requirement in the safety evaluation of industrial and consumer products. None in vitro ocular irritation assays are capable of fully categorizing chemicals as stand-alone. Therefore, the CEFIC-LRI-AIMT6-VITO CON4EI consortium assessed the reliability of eight in vitro test methods and computational models as well as established a tiered-testing strategy. One of the selected assays was Bovine Corneal Opacity and Permeability (BCOP). In this project, the same corneas were used for measurement of opacity using the OP-KIT, the Laser Light-Based Opacitometer (LLBO) and for histopathological analysis. The results show that the accuracy of the BCOP OP-KIT in identifying Cat 1 chemicals was 73.8% while the accuracy was 86.3% for No Cat chemicals. BCOP OP-KIT false negative results were often related to an in vivo classification driven by conjunctival effects only. For the BCOP LLBO, the accuracy in identifying Cat 1 chemicals was 74.4% versus 88.8% for No Cat chemicals. The BCOP LLBO seems very promising for the identification of No Cat liquids but less so for the identification of solids. Histopathology as an additional endpoint to the BCOP test method does not reduce the false negative rate substantially for in vivo Cat 1 chemicals. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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Full Text from ScienceDirect