Crystallization and preliminary X-ray analysis of pyruvate kinase type I from Escherichia coli
| Autor: | Andrea Mattevi, M L Speranza, Martino Bolognesi, P. Sartori, Alessandro Coda, Giovanna Valentini |
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| Rok vydání: | 1995 |
| Předmět: |
chemistry.chemical_classification
Chemistry education Resolution (electron density) General Medicine Pyruvate dehydrogenase phosphatase medicine.disease_cause law.invention Crystallography Enzyme Biochemistry Structural Biology law medicine Molecular symmetry Crystallization X ray analysis Escherichia coli Pyruvate kinase |
| Zdroj: | Acta crystallographica. Section D, Biological crystallography. 51(Pt 6) |
| ISSN: | 0907-4449 |
| Popis: | Crystals of the fructose-l,6-bisphosphate-dependent pyruvate kinase from Escherichia coli have been grown using the hanging-drop vapour-diffusion technique. The space group was found to be C222(1) with cell dimensions a = 76.8, b = 247.5, c = 132.6 A. Diffraction data to 3.0 A resolution have been recorded and the enzyme molecular symmetry analysed through inspection of the self-rotation function. The crystallized protein is in the allosterically inactive T state. |
| Databáze: | OpenAIRE |
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