Genetic characterisation of influenza A(H1N1)pdm09 viruses circulating in Assam, Northeast India during 2009–2015

Autor: Mousumi Dutta, Dipankar Biswas, Manika Buragohain, Kaushal Yadav, Kimmi Sarmah, Kishore Sarma, Biswajyoti Borkakoty
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0301 basic medicine
Male
viruses
lcsh:QR1-502
influenza A(H1N1)pdm09
Hemagglutinin Glycoproteins
Influenza Virus
neuraminidase
lcsh:Microbiology
chemistry.chemical_compound
0302 clinical medicine
Influenza A Virus
H1N1 Subtype
Immunology and Microbiology (miscellaneous)
Pandemic
Immunology and Allergy
030212 general & internal medicine
Northeast India
Child
Phylogeny
Haemagglutinin
Aged
80 and over
Respiratory tract infections
biology
virus diseases
Middle Aged
Infectious Diseases
Child
Preschool
RNA
Viral
Female
Microbiology (medical)
Adult
Oseltamivir
Adolescent
030106 microbiology
Immunology
Virulence
India
Real-Time Polymerase Chain Reaction
Microbiology
Antiviral Agents
Virus
03 medical and health sciences
Young Adult
Influenza
Human
Humans
Aged
General Immunology and Microbiology
Molecular epidemiology
Base Sequence
Sequence Analysis
RNA
Influenza A Virus
H3N2 Subtype
pandemic
Outbreak
Genetic Variation
Infant
Virology
chemistry
biology.protein
Neuraminidase
Zdroj: Indian Journal of Medical Microbiology, Vol 37, Iss 1, Pp 42-49 (2019)
ISSN: 1998-3646
0255-0857
Popis: Introduction: Influenza A(H1N1)pdm09 virus, since its identification in April 2009, has continued to cause significant outbreaks of respiratory tract infections including pandemics in humans. In the course of its evolution, the virus has acquired many mutations with an ability to cause increased disease severity. A regular molecular surveillance of the virus is essential to mark the evolutionary changes that may cause a shift to the viral behavior. Materials and Methods: Samples of Throat/Nasal swabs were collected from a total of 3715 influenza-like illness cases and screened by Real-time Reverse Transcription-Polymerase Chain Reaction for influenza viruses. Nucleotide sequence analysis was done to identify changes in antigenicity of the virus strains. Results: The present study describes the molecular characteristics of influenza A(H1N1)pdm09 viruses detected in Assam of Northeast India during 2009–2015. Influenza A viruses were detected in 11.4% (425/3715), of which influenza A(H1N1)pdm09 viruses were detected in 41.4% (176/425). The nucleotide sequencing of influenza A(H1N1)pdm09 viruses revealed a total of 17 and 22 amino acid substitutions in haemagglutinin (HA) and neuraminidase (NA) genes of the virus, respectively, compared to contemporary vaccine strain A/California/07/2009. The important mutations detected in HA genes of A/Assam(H1N1)pdm09 strains included E391K, K180Q and S202T. Mutation 'N248D' which has an ability to develop oseltamivir resistance was also detected in NA gene of A/Assam(H1N1)pdm09 strains. Conclusions: Regular molecular surveillance of influenza A(H1N1)pdm09 is important to monitor the viral behavior in terms of increase virulence, drug resistance pattern and emergence of novel strains.
Databáze: OpenAIRE
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