Polymorphisms in the bovine tumour necrosis factor receptor type two gene (TNF-RII) and cell subpopulations naturally infected with bovine leukaemia virus
| Autor: | B. Bojarojc-Nosowicz, Alicja Stachura, Dariusz Kaczmarczyk, E. Kaczmarczyk |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
040301 veterinary sciences Veterinary medicine viruses BLV Biology Immunofluorescence Virus 0403 veterinary science 03 medical and health sciences Polymorphism (computer science) SF600-1100 Genotype medicine IgM+ TNFα+p24+ cells Gene General Veterinary medicine.diagnostic_test CD11b+TNFα+p24+ cells 04 agricultural and veterinary sciences Molecular biology 030104 developmental biology cattle microsatellite DNA Microsatellite Tumor necrosis factor alpha Nested polymerase chain reaction Research Article |
| Zdroj: | Journal of Veterinary Research Journal of Veterinary Research, Vol 63, Iss 2, Pp 175-182 (2019) |
| ISSN: | 2450-8608 |
| Popis: | Introduction: Numerous mutations in the bovine tumour necrosis factor receptor type two (TNF-RII) gene have been identified, but their biological consequences remain poorly understood. The aim of this study was to determine whether polymorphism in the analysed loci of the bovine TNF-RII gene is linked with the size of cell subpopulations naturally infected with bovine leukaemia virus (BLV) which serve important immune functions in the host. Material and Methods: Samples originated from 78 cows. Polymorphisms in the studied gene were determined by PCR-RFLP and DNA sequencing by capillary electrophoresis. BLV infection was diagnosed by the immunofluorescence (IMF) technique and nested PCR. Cell subpopulations were immunophenotyped with IMF. Results: Similar and non-significant differences in the average percentages of TNFα+, IgM+TNFα+, and CD11b+TNFα+ cells infected with BLV were noted in individuals with various genotypes in the polymorphic sites g.-1646T > G and g.16534T > C of the TNF-RII gene, and significant differences in the percentages of these subpopulations were observed between selected microsatellite genotypes (g.16512CA(n)). Conclusion: STR polymorphism and the number of CA dinucleotide repeats in intron 1 of the TNF-RII gene influence the frequency of TNF+, CD11b+TNF+, and IgM+TNF+ subpopulations naturally infected with BLV. Polymorphism in the gene’s other two sites do not affect the size of these cell subpopulations. |
| Databáze: | OpenAIRE |
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