Decreased TUSC3 Promotes Pancreatic Cancer Proliferation, Invasion and Metastasis

Autor: Zhuonan Zhuang, Xiu Zhang, Yunfei Wang, Yong’an Chen, Xiaolong Deng, Hua Feng, Xuetao Yu, Xiaoqiang Fan, Long Peng, Jie Shen, Haibin Zhao
Rok vydání: 2016
Předmět:
Male
0301 basic medicine
Pathology
Protein Expression
lcsh:Medicine
Gene Expression
Suppressor Genes
Metastasis
0302 clinical medicine
Basic Cancer Research
Gene expression
Medicine and Health Sciences
lcsh:Science
Staining
Mice
Inbred BALB C
Multidisciplinary
Liver Neoplasms
NF-kappa B
Cell Staining
Animal Models
Middle Aged
Prognosis
Ovarian Cancer
Oncology
Gene Knockdown Techniques
Lymphatic Metastasis
030220 oncology & carcinogenesis
Female
CA19-9
Research Article
Adult
medicine.medical_specialty
Tumor suppressor gene
Tumor Suppressor Genes
Mice
Nude
Mouse Models
Biology
Research and Analysis Methods
Disease-Free Survival
Pancreatic Cancer
03 medical and health sciences
Model Organisms
Gene Types
Cell Line
Tumor
Pancreatic cancer
Gastrointestinal Tumors
Biomarkers
Tumor
Genetics
Gene Expression and Vector Techniques
medicine
Animals
Humans
Neoplasm Invasiveness
RNA
Messenger
Molecular Biology Techniques
Molecular Biology
Aged
Cell Proliferation
Proportional Hazards Models
Molecular Biology Assays and Analysis Techniques
Cell growth
Tumor Suppressor Proteins
lcsh:R
Membrane Proteins
Correction
Cancers and Neoplasms
Biology and Life Sciences
medicine.disease
Pancreatic Neoplasms
030104 developmental biology
Specimen Preparation and Treatment
Cell culture
Multivariate Analysis
Cancer research
lcsh:Q
Neoplasm Recurrence
Local
Ovarian cancer
Gynecological Tumors
Neoplasm Transplantation
Zdroj: PLoS ONE
PLoS ONE, Vol 11, Iss 2, p e0149028 (2016)
ISSN: 1932-6203
Popis: Pancreatic cancer is an aggressive disease with dismal prognosis. It is of paramount importance to understand the underlying etiological mechanisms and identify novel, consistent, and easy-to-apply prognostic factors for precision therapy. TUSC3 (tumor suppressor candidate 3) was identified as a potential tumor suppressor gene and previous study showed TUSC3 is decreased in pancreatic cancer at mRNA level, but its putative tumor suppressor function remains to be verified. In this study, TUSC3 expression was found to be suppressed both at mRNA and protein levels in cell line models as well as in clinical samples; decreased TUSC3 expression was associated with higher pathological TNM staging and poorer outcome. In three pairs of cell lines with different NF-κB activity, TUSC3 expression was found to be reversely correlated with NF-κB activity. TUSC3-silenced pancreatic cancer cell line exhibited enhanced potential of proliferation, migration and invasion. In an orthotopic implanted mice model, TUSC3 silenced cells exhibited more aggressive phenotype with more liver metastasis. In conclusion, the current study shows that decreased immunological TUSC3 staining is a factor prognostic of poor survival in pancreatic cancer patients and decreased TUSC3 promotes pancreatic cancer cell proliferation, invasion and metastasis. The reverse correlation between NF-κB activity and TUSC3 expression may suggest a novel regulation pattern for this molecule.
Databáze: OpenAIRE
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