Decreased TUSC3 Promotes Pancreatic Cancer Proliferation, Invasion and Metastasis
Autor: | Zhuonan Zhuang, Xiu Zhang, Yunfei Wang, Yong’an Chen, Xiaolong Deng, Hua Feng, Xuetao Yu, Xiaoqiang Fan, Long Peng, Jie Shen, Haibin Zhao |
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Rok vydání: | 2016 |
Předmět: |
Male
0301 basic medicine Pathology Protein Expression lcsh:Medicine Gene Expression Suppressor Genes Metastasis 0302 clinical medicine Basic Cancer Research Gene expression Medicine and Health Sciences lcsh:Science Staining Mice Inbred BALB C Multidisciplinary Liver Neoplasms NF-kappa B Cell Staining Animal Models Middle Aged Prognosis Ovarian Cancer Oncology Gene Knockdown Techniques Lymphatic Metastasis 030220 oncology & carcinogenesis Female CA19-9 Research Article Adult medicine.medical_specialty Tumor suppressor gene Tumor Suppressor Genes Mice Nude Mouse Models Biology Research and Analysis Methods Disease-Free Survival Pancreatic Cancer 03 medical and health sciences Model Organisms Gene Types Cell Line Tumor Pancreatic cancer Gastrointestinal Tumors Biomarkers Tumor Genetics Gene Expression and Vector Techniques medicine Animals Humans Neoplasm Invasiveness RNA Messenger Molecular Biology Techniques Molecular Biology Aged Cell Proliferation Proportional Hazards Models Molecular Biology Assays and Analysis Techniques Cell growth Tumor Suppressor Proteins lcsh:R Membrane Proteins Correction Cancers and Neoplasms Biology and Life Sciences medicine.disease Pancreatic Neoplasms 030104 developmental biology Specimen Preparation and Treatment Cell culture Multivariate Analysis Cancer research lcsh:Q Neoplasm Recurrence Local Ovarian cancer Gynecological Tumors Neoplasm Transplantation |
Zdroj: | PLoS ONE PLoS ONE, Vol 11, Iss 2, p e0149028 (2016) |
ISSN: | 1932-6203 |
Popis: | Pancreatic cancer is an aggressive disease with dismal prognosis. It is of paramount importance to understand the underlying etiological mechanisms and identify novel, consistent, and easy-to-apply prognostic factors for precision therapy. TUSC3 (tumor suppressor candidate 3) was identified as a potential tumor suppressor gene and previous study showed TUSC3 is decreased in pancreatic cancer at mRNA level, but its putative tumor suppressor function remains to be verified. In this study, TUSC3 expression was found to be suppressed both at mRNA and protein levels in cell line models as well as in clinical samples; decreased TUSC3 expression was associated with higher pathological TNM staging and poorer outcome. In three pairs of cell lines with different NF-κB activity, TUSC3 expression was found to be reversely correlated with NF-κB activity. TUSC3-silenced pancreatic cancer cell line exhibited enhanced potential of proliferation, migration and invasion. In an orthotopic implanted mice model, TUSC3 silenced cells exhibited more aggressive phenotype with more liver metastasis. In conclusion, the current study shows that decreased immunological TUSC3 staining is a factor prognostic of poor survival in pancreatic cancer patients and decreased TUSC3 promotes pancreatic cancer cell proliferation, invasion and metastasis. The reverse correlation between NF-κB activity and TUSC3 expression may suggest a novel regulation pattern for this molecule. |
Databáze: | OpenAIRE |
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