Paradoxically decreased intracellular collagenase activity in macrophages from bronchoalveolar lavage fluid in current smokers and patients with obstructive ventilatory disorder
| Autor: | Hirotsugu Ohkubo, Tsuyoshi Yoshida, Yoshikazu Ito, Yusuke Hakoda, Kazuma Ohyashiki, Masahiro Aoshima, Hidekazu Tago, Kenta Utsumi, Kazushige Minemura |
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| Rok vydání: | 2004 |
| Předmět: |
Adult
medicine.medical_specialty CD14 Lipopolysaccharide Receptors Matrix metalloproteinase Pulmonary Disease Chronic Obstructive FEV1/FVC ratio Internal medicine Macrophages Alveolar Genetics Humans Medicine Collagenases Respiratory system Aged COPD medicine.diagnostic_test business.industry Smoking General Medicine Middle Aged medicine.disease respiratory tract diseases Enzyme Activation Endocrinology Bronchoalveolar lavage Immunology Collagenase Leukocyte Common Antigens business Bronchoalveolar Lavage Fluid Intracellular medicine.drug |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1791-244X 1107-3756 |
| DOI: | 10.3892/ijmm.13.6.859 |
| Popis: | The roles of matrix metalloproteinases (MMPs) in chronic obstructive pulmonary disease (COPD) have been studied. Macrophages are considered to release MMPs in the lung tissue. We measured intracellular collagenase activity in intact CD14(+)CD45++ cells from the bronchoalveolar lavage fluid (BALF) of patients with obstructive ventilatory impairment and other respiratory diseases. Collagenase activity in current smokers was significantly lower than those in non-smokers (3.25+/-0.54 versus 5.48+/-0.55; P=0.006), and also lower than those in ex-smokers (versus 6.54+/-1.54; P=0.019). We found a lower activity of collagenase in patients with FEV1/FVC below 70% than those with 70% or higher (2.68+/-0.59 versus 4.51+/-0.44; P=0.034). Mean value of FEV1/FVC in patients with collagenase activity of 4 or higher was significantly elevated as compared to those with the activity lower than 4 (83.3+/-3.3 versus 71.8+/-4.9; P=0.021). The discrepancy between increased release of MMPs by previous reports and decreased intracellular activity in our results, may be explained by the production of inactive form of MMPs is relatively increased in COPD. Our study may provide the future direction in investigating the mechanism of COPD. In clinics, this measurement in patients with smoking habits may be helpful to advise them to stop smoking, and to avoid progression to the irreversible obstructive disease. |
| Databáze: | OpenAIRE |
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