Rapid production of specific vaccines for lymphoma by expression of the tumor-derived single-chain Fv epitopes in tobacco plants
| Autor: | Kathleen M. Hanley, Thomas H. Turpen, Laurence K. Grill, Ronald Levy, Alison A. McCormick, Monto H. Kumagai, Itzhak Hakim, Shoshana Levy, Daniel Tuse |
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| Rok vydání: | 1999 |
| Předmět: |
Glycosylation
Lymphoma medicine.medical_treatment Genetic Vectors Molecular Sequence Data Nicotiana benthamiana chemical and pharmacologic phenomena Biology Epitope Epitopes Mice Affinity chromatography Gene Expression Regulation Plant Conjugate vaccine Tobacco Tumor Cells Cultured medicine Animals Amino Acid Sequence Cloning Molecular B-cell lymphoma Immunoglobulin Fragments Plant Proteins B-Lymphocytes Vaccines Multidisciplinary Base Sequence Tobamovirus Vaccination Hemocyanin Neoplasms Experimental Biological Sciences medicine.disease biology.organism_classification Virology Molecular biology Blot Plants Toxic biology.protein Antibody |
| Zdroj: | Proceedings of the National Academy of Sciences. 96:703-708 |
| ISSN: | 1091-6490 0027-8424 |
| Popis: | Rapid production of protein-based tumor-specific vaccines for the treatment of malignancies is possible with the plant-based transient expression system described here. We created a modified tobamoviral vector that encodes the idiotype-specific single-chain Fv fragment (scFv) of the immunoglobulin from the 38C13 mouse B cell lymphoma. InfectedNicotiana benthamianaplants contain high levels of secreted scFv protein in the extracellular compartment. This material reacts with an anti-idiotype antibody by Western blotting, ELISA, and affinity chromatography, suggesting that the plant-produced 38C13 scFv protein is properly folded in solution. Mice vaccinated with the affinity-purified 38C13 scFv generate >10 μg/ml anti-idiotype immunoglobulins. These mice were protected from challenge by a lethal dose of the syngeneic 38C13 tumor, similar to mice immunized with the native 38C13 IgM-keyhole limpet hemocyanin conjugate vaccine. This rapid production system for generating tumor-specific protein vaccines may provide a viable strategy for the treatment of non-Hodgkin’s lymphoma. |
| Databáze: | OpenAIRE |
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