The effects of a novel non-invasive application of platelet-rich fibrin on periodontal clinical parameters and gingival crevicular fluid transforming growth factor-β and collagen-1 levels: A randomized, controlled, clinical study
| Autor: | Muhittin Serdar, Erkan Özcan, Taner Ozgurtas, Alpdogan Kantarci, Savaş Özarslantürk, Isil Saygun |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Gingival and periodontal pocket Gastroenterology Fibrin Collagen Type I Root Planing 03 medical and health sciences 0302 clinical medicine Scaling and root planing Transforming Growth Factor beta Internal medicine Platelet-Rich Fibrin medicine Humans Gingival recession Periodontitis biology business.industry 030206 dentistry Gingival Crevicular Fluid medicine.disease Platelet-rich fibrin Collagen type I alpha 1 030104 developmental biology biology.protein Periodontics Dental Scaling medicine.symptom Periodontal Index business Transforming growth factor |
| Zdroj: | Journal of periodontologyREFERENCES. 92(9) |
| ISSN: | 1943-3670 |
| Popis: | Background Several potential benefits have been attributed to the platelet-rich fibrin (PRF), including enhanced tissue healing properties. In this study, we hypothesized that the application of PRF as an adjunct to conventional scaling and root planing (ScRp) would enhance the outcomes of non-surgical periodontal therapy. Methods The present study was a split-mouth randomized controlled clinical trial design in 24 deep periodontal pockets in 12 patients with periodontitis. The pockets were randomly assigned as test or control. The test group received PRF as an adjunct to ScRp, whereas the control group received ScRp only. We measured periodontal clinical parameters at baseline, 3, and 6 months after the treatments. To study the initial healing in response to treatment, transforming growth factor-β (TGF-β) and collagen-1 (Col-1) in gingival crevicular fluid (GCF) were measured using enzyme-linked immunosorbent assay at baseline, third, seventh, and 14th days. Results The test group showed a significantly greater pocket reduction, higher clinical attachment gain, and less gingival recession than the control group at 3 and 6 months. The test Col-1 levels (1.27 ± 1.05, 1.35 ± 0.76, 0.97 ± 0.53 ng/site) and TGF-β levels (11.93 ± 2.68, 12.54 ± 3.66, 17.19 ± 11.66 pg/site) were higher than the control Col-1 levels (0.76 ± 0.20, 0.84 ± 0.24, 0.57 ± 0.19 ng/site) and TGF-β levels (6.34 ± 1.67, 6.35 ± 3.44, 7.51 ± 2.85 pg/site) at all measurement days respectively. Conclusions Non-surgical application of the PRF as an adjunct to conventional ScRp may effectively improve the periodontal clinical parameters via increasing expression of the GCF TGF-β and Col-1 levels. |
| Databáze: | OpenAIRE |
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