High-resolution integrated map encompassing the breast cancer loss of heterozygosity region on human chromosome 16q22.1
| Autor: | Sergey Shaposhnikov, Henning Johansen, Michel Longy, Françoise Dorion-Bonnet, Kaja Klykken Aas, Hans Prydz, Jim Thorsen, Martin Krekling, Laurence Taine, Delfine Lafon, Christophe Bepoldin, Azza Abd El Monéim, Philippe Rocca-Serra, Eirik Frengen |
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| Rok vydání: | 2001 |
| Předmět: |
Genetic Markers
Molecular Sequence Data Restriction Mapping Loss of Heterozygosity Breast Neoplasms Biology Polymerase Chain Reaction CDH1 Loss of heterozygosity Chromosome 16 Breast cancer Gene mapping Genetics medicine Humans Genes Tumor Suppressor Contig medicine.diagnostic_test Ductal carcinoma medicine.disease Physical Chromosome Mapping biology.protein Chromosomes Human Pair 16 Fluorescence in situ hybridization Microsatellite Repeats |
| Zdroj: | Genomics. 70(3) |
| ISSN: | 0888-7543 |
| Popis: | Loss of heterozygosity (LOH) on the long arm of human chromosome 16 is a common genetic alteration observed in both invasive ductal and invasive lobular breast carcinomas. We have generated a high-resolution integrated map encompassing the smallest region of LOH overlap within chromosome 16q22.1 (SRO2). Southern hybridization experiments using more than 140 probes resulted in the assembly of 152 bacterial large-insert clones into a 2.8-Mb contig covering SRO2. The structure of the contig was verified by long-range mapping using total human genomic DNA, and the contig orientation was determined by fluorescence in situ hybridization. A total of 68 transcripts have been identified in the map. One of the genes residing within SRO2 is the E-cadherin gene, CDH1, which has previously been shown to be mutated in lobular breast carcinomas, resulting in loss of E-cadherin expression. In most cases of ductal carcinoma, which is the major mammary cancer type, E-cadherin is normally expressed, suggesting that other genes within 16q22.1 are involved in the development of this tumor subtype. The high-resolution map presented in this study provides a valuable resource for identification of tumor suppressor genes expected to be involved in the etiology of breast carcinomas. |
| Databáze: | OpenAIRE |
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