Immunization with MHC class I-negative but not -positive HPV16-associated tumour cells inhibits growth of MHC class I-negative tumours
| Autor: | Milan Reinis, Marie Indrová, Jan Bubeník, Jana Símová, Simona Moravcova, Jana Bieblová, Hana Pribylova, Jandlová T |
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| Rok vydání: | 2007 |
| Předmět: |
Human papillomavirus 16
Cancer Research biology Antigen processing Histocompatibility Antigens Class I CD1 chemical and pharmacologic phenomena C-C chemokine receptor type 7 Cross Reactions MHC restriction Cancer Vaccines Virus Mice Inbred C57BL Mice Immune system Oncology Cell culture Cell Line Tumor Neoplasms MHC class I Immunology biology.protein Animals Immunization Cell Proliferation |
| Zdroj: | International Journal of Oncology. |
| ISSN: | 1791-2423 1019-6439 |
| DOI: | 10.3892/ijo.30.4.1011 |
| Popis: | Loss or downregulation of MHC class I molecules on tumour cells is a common mechanism by which tumours can escape from T-cell mediated immune responses. In this study we have investigated the immunologic crossreactivity between murine tumour cell lines expressing human papilloma virus (HPV) 16-derived E6/E7 oncoproteins with distinct surface expression of MHC class I molecules. The aims of this study were to demonstrate whether immune responses capable of coping with MHC class I-positive tumours can also be effective against their MHC class I-deficient derivatives and whether it is possible to induce immunity against MHC class I-deficient tumours by cellular vaccines based on MHC class I-deficient tumour cell lines. Our data showed that immunization with MHC class I-deficient but not with MHC class I positive tumour cells inhibited the growth of MHC class I-deficient tumours. In vivo depletion studies revealed that the mechanisms underlying effective immune responses against MHC class I-negative tumours in animals immunized with MHC class I-deficient tumour cells involved natural killer cells. The presented findings are of particular clinical relevance in the sense of construction of vaccines directed against a broad spectrum of HPV-associated tumours. |
| Databáze: | OpenAIRE |
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