Examining Trichophyton tonsurans genotype and biochemical phenotype as determinants of disease severity in tinea capitis
| Autor: | Susan M. Abdel-Rahman, Nasreen Talib, Amy J. Nopper, Gerald J. Wyckoff, Ada Solidar |
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| Rok vydání: | 2008 |
| Předmět: |
Male
Genotype medicine.disease_cause Polymorphism Single Nucleotide Severity of Illness Index Fungal Proteins Trichophyton Severity of illness DNA Ribosomal Spacer medicine Humans Child DNA Fungal Genotyping Tinea Capitis Trichophyton tonsurans Phylogeny biology Fungal genetics Proteolytic enzymes General Medicine biology.organism_classification medicine.disease Infectious Diseases Phenotype Child Preschool Immunology Dermatophyte Tinea capitis Female Peptide Hydrolases |
| Zdroj: | Medical mycology. 46(3) |
| ISSN: | 1369-3786 |
| Popis: | Trichophyton tonsurans infections occur in various host populations, on various body sites and with varying degrees of inflammation. This investigation was undertaken to determine whether fungal factors could explain the degree of severity in clinical symptomatology among infected children. Otherwise healthy children (n=54) presenting with tinea capitis were enrolled in this study. A thorough history was performed, the extent and severity of infection graded and a fungal specimen collected from each child. Strain type was determined by genotyping for 11 sequence variations in the rDNA and ALP1 loci. Secreted protease activity was quantitated after 5 days of growth in aqueous medium. Forty participants were evaluable. Infection duration ranged from 1 day to 3 years and clinical severity score (CSS) from 4-19. Seventeen unique fungal genotypes were present. Keratinase, collagenase and elastase activity varied 32.7-fold, 64.9-fold and 303.3-fold, respectively. A significant association was observed between genotype and disease severity with the rDNA sequence variations accounting for over 50% of the variation observed in CSS (r2=0.539; P |
| Databáze: | OpenAIRE |
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