Affinity and catalytic heterogeneity of polyclonal myelin basic protein-hydrolyzing IgGs from sera of patients with multiple sclerosis
| Autor: | Georgy A. Nevinsky, Galina A. Legostaeva, Dar’ya I. Polosukhina, Anna M. Bezuglova, Boris M. Doronin, Valentina N. Buneva |
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| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Adult
Male Multiple Sclerosis Adolescent Blotting Western Antibody Affinity catalytic IgG hydrolysis of myelin basic protein Enzyme-Linked Immunosorbent Assay Immunoglobulin light chain Immunoglobulin G Young Adult medicine Humans human blood Autoantibodies Oligopeptide biology Chemistry Multiple sclerosis Hydrolysis Autoantibody Antibodies Monoclonal Myelin Basic Protein Cell Biology Articles Hydrogen-Ion Concentration Middle Aged medicine.disease Myelin basic protein Kinetics Biochemistry Polyclonal antibodies biology.protein Biocatalysis Molecular Medicine Female Antibody Peptide Hydrolases |
| Zdroj: | Journal of Cellular and Molecular Medicine |
| ISSN: | 1582-4934 1582-1838 |
| Popis: | Human myelin basic protein (hMBP)-hydrolyzing activity was recently shown to be an intrinsic property of antibodies (Abs) from multiple sclerosis (MS) patients. Here, we present the first evidence demonstrating a significant diversity of different fractions of polyclonal IgGs (pIgGs) from MS patients in their affinity for hMBP and in the ability of pIgGs to hydrolyze hBMP at different optimal pHs (3–10.5). IgGs containing λ- and κ-types of light chains demonstrated comparable relative activities in the hydrolysis of hMBP. IgGs of IgG1–IgG4 sub-classes were analyzed for catalytic activity. IgGs of all four sub-classes were catalytically active, with their contribution to the total activity of Abzs in the hydrolysis of hMBP and its 19-mer oligopeptide increasing in the order: IgG1 (1.5–2.1%) < IgG2 (4.9–12.8%) < IgG3 (14.7–25.0%) < IgG4 (71–78%). Our findings suggest that the immune systems of individual MS patients generate a variety of anti-hMBP abzymes with different catalytic properties, which can attack hMBP of myelin-proteolipid shell of axons, playing an important role in MS pathogenesis. |
| Databáze: | OpenAIRE |
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