Endogenous conversion of n-6 to n-3 polyunsaturated fatty acids facilitates the repair of cardiotoxin-induced skeletal muscle injury in fat-1 mice
| Autor: | Shuang Liang, Peng Cheng, Qing Yang, Zi-Qing Zhu, Anmin Chen, Zheng-gang Wang, Zhi-yi He |
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| Rok vydání: | 2021 |
| Předmět: |
Fatty Acid Desaturases
Aging medicine.medical_specialty injury n-3 PUFAs Mice Transgenic fat-1 mice Cardiotoxins Mice Gastrocnemius muscle Cardiotoxin Fatty Acids Omega-6 Internal medicine Fatty Acids Omega-3 medicine Animals Regeneration Myocyte skeletal muscle Caenorhabditis elegans Proteins Muscle Skeletal chemistry.chemical_classification biology Chemistry Skeletal muscle Cell Biology Eicosapentaenoic acid Fatty acid desaturase medicine.anatomical_structure Endocrinology biology.protein C2C12 Research Paper Polyunsaturated fatty acid |
| Zdroj: | Aging (Albany NY) |
| ISSN: | 1945-4589 |
| DOI: | 10.18632/aging.202655 |
| Popis: | In this study, we investigated the beneficial effects of high endogenous levels of n-3 polyunsaturated fatty acids (PUFAs) on skeletal muscle repair and regeneration using a mouse cardiotoxin (CTX, 20 μM/200 μL) -induced gastrocnemius muscle injury model. Transgenic fat-1 mice expressing the Caenorhabditis elegans fat-1 gene, encoding n-3 fatty acid desaturase, showed higher n-3 PUFA levels and lower n-6/n-3 PUFA ratios in gastrocnemius muscle tissues. Hematoxylin and eosin and Masson’s trichrome staining of gastrocnemius sections revealed increased muscle fiber size and reduced fibrosis in fat-1 mice on days 7 and 14 after CTX injections. Gastrocnemius muscle tissues from fat-1 mice showed reduced inflammatory responses and increased muscle fiber regeneration reflecting enhanced activation of satellite cells on day 3 after cardiotoxin injections. Gastrocnemius muscle tissues from cardiotoxin-treated fat-1 mice showed reduced levels of pro-apoptotic proteins (Caspase 3 and Bax) and increased levels of anti-apoptotic proteins (Bcl-2 and Survivin). Moreover, eicosapentaenoic acid (EPA) reduced the incidence of apoptosis among cardiotoxin-treated C2C12 mouse myoblasts. These findings demonstrate that higher endogenous n-3 PUFA levels in fat-1 mice enhances skeletal muscle repair and regeneration following cardiotoxin-induced injury. |
| Databáze: | OpenAIRE |
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