High-fat and high-cholesterol diet decreases phosphorylated inositol-requiring kinase-1 and inhibits autophagy process in rat liver
| Autor: | Yuki Yoshikawa-Bando, Hisao Naito, Yuan Yuan, Kazuya Kitamori, Sayuki Hashimoto, Hiroshi Yatsuya, Tamie Nakajima |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Chaperone-mediated autophagy Science Protein degradation Protein Serine-Threonine Kinases Immunoglobulin light chain Diet High-Fat Article Cholesterol Dietary 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Multienzyme Complexes Non-alcoholic Fatty Liver Disease Internal medicine Endoribonucleases medicine Autophagy Animals Humans Inositol Phosphorylation Non-alcoholic steatohepatitis Multidisciplinary Kinase Chemistry Endoplasmic reticulum medicine.disease Endoplasmic Reticulum Stress Rats Disease Models Animal 030104 developmental biology Endocrinology Liver Medicine Steatohepatitis Microtubule-Associated Proteins 030217 neurology & neurosurgery |
| Zdroj: | Scientific Reports Scientific Reports, Vol 9, Iss 1, Pp 1-9 (2019) |
| ISSN: | 2045-2322 |
| Popis: | Precise molecular pathways involved in the progression of non-alcoholic steatohepatitis (NASH) remain to be elucidated. As Mallory–Denk bodies were occasionally observed in the enlarged hepatocytes in NASH model rat (SHRSP5/Dmcr) fed high-fat and high-cholesterol (HFC) diet, we aimed to clarify the roles of autophagy and endoplasmic reticulum (ER) stress in NASH progression. Male SHRSP5/Dmcr were randomly divided into 4 groups. Two groups were fed a control diet; the other two groups were fed a HFC diet for 2 and 8 weeks, respectively. The HFC diet increased the autophagy-related proteins levels and microtubule-associated protein 1 light chain 3-II/I ratio after 2 and 8 weeks, respectively. However, regarding ER stress-related proteins, the HFC diet decreased the levels of phosphorylated (p-) inositol-requiring kinase-1 (p-IRE-1) and p-protein kinase RNA-like ER kinase after 2 weeks. Additionally, the HFC diet increased anti-ubiquitin-positive cells and the level of the autophagy substrate p62, suggesting that the HFC diet induced dysfunction in ubiquitin-dependent protein degradation pathways. In conclusion, the HFC diet arrested the autophagy process in the liver; this was particularly associated with decreases in p-IRE-1 expression. |
| Databáze: | OpenAIRE |
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