Search for phase specific genes in the brain of desert locust, Schistocerca gregaria (Orthoptera: Acrididae) by differential display polymerase chain reaction
| Autor: | A. De Loof, Murshida Begum, Roger Huybrechts, Michael Breuer, A Vandingenen, Mazibur M. Rahman |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Genetics
Differential display DNA Complementary Base Sequence biology Protein family Physiology Gene Expression Profiling Molecular Sequence Data Brain Grasshoppers biology.organism_classification Polymerase Chain Reaction Biochemistry Molecular biology Acrididae Gene expression Animals Gene family Schistocerca Amino Acid Sequence Primer (molecular biology) Sequence Alignment Molecular Biology Locust |
| Zdroj: | Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology. 135:221-228 |
| ISSN: | 1095-6433 |
| Popis: | Differential display reverse transcriptase polymerase chain reaction (DDRT-PCR) in combination with semi-quantitative RT-PCR was used to compare differences in gene expression between the solitary and gregarious phase of Schistocerca gregaria. Twenty-six primer combinations were used, which produced 8 differential bands. Two out of the 8 differentials, one typical for the solitary and one for the gregarious phase, were further analyzed by semi-quantitative RT-PCR. The expression level of the solitary phase specific gene (SSG) was 2 times higher in solitary animals as compared to gregarious ones, while the gregarious specific gene (GSG) gave a 4-fold higher expression level in gregarious animals than in solitaries. Sequence analysis demonstrated that SSG does not belong to a known gene family, while the GSG belongs to the SPARC protein family. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect