In vitro cow uterine response to Escherichia coli, leukotrienes and cytokines
| Autor: | E. Piotrowicz, A.A. Szczepańska, B.M. Socha, M. Łupicka, W. Barański, Anna Korzekwa |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Leukotrienes medicine.medical_specialty Immunology Cattle Diseases Prostaglandin Inflammation In Vitro Techniques Biology medicine.disease_cause 03 medical and health sciences chemistry.chemical_compound Internal medicine Escherichia coli medicine Animals RNA Messenger Receptor Escherichia coli Infections General Veterinary Interleukin-6 Uterus Interleukin Puerperal Disorders Molecular biology In vitro Toll-Like Receptor 4 030104 developmental biology Endocrinology chemistry Prostaglandins TLR4 Cytokines Cattle Female lipids (amino acids peptides and proteins) Tumor necrosis factor alpha medicine.symptom Endometritis |
| Zdroj: | Veterinary Immunology and Immunopathology. 182:59-62 |
| ISSN: | 0165-2427 |
| DOI: | 10.1016/j.vetimm.2016.10.001 |
| Popis: | The aim was to determine the dynamic profile of interactions between Escherichia coli (E. coli) and the actions of leukotrienes (LTs) and TNF and INFγ (cytokines) in the uterus in vitro. Uterine explants (N=6) were incubated for 2, 12 and 24h either as E. coli-treated (106CFU) or non-treated and/or with: LTB4 and C4 (10-6M, for both LTs), LTs receptors antagonists (aLTR; 10-6M) and/or cytokines (each 10ng/ml). Toll Like Receptor 4 (TLR4) mRNA expression increased in explants incubated with E. coli, cytokines and LTs after 2 and 12h and aLTR inhibited the effect of LTs in explants incubated with E. coli (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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