A novel testis-specific Na H exchanger is involved in sperm motility and fertility
| Autor: | Xue-Sen Zhang, Zhao-Yuan Hu, Wu-Lin Zuo, Huan Cai, Jun-Cheng Huang, Wen-Liang Zhou, Cui-Ling Lu, Tao Liu, Fei Gao, Yi-Xun Liu, Min Chen, Yi-Chuan Li |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Male
DNA Complementary Sodium-Hydrogen Exchangers Intracellular pH Blotting Western Molecular Sequence Data Acrosome reaction Motility Enzyme-Linked Immunosorbent Assay General Biochemistry Genetics and Molecular Biology Mice Capacitation Testis Animals Humans Cloning Molecular In Situ Hybridization Sperm motility DNA Primers Genetics Analysis of Variance Mice Inbred BALB C Microscopy Confocal Base Sequence General Immunology and Microbiology Sperm flagellum biology Sequence Analysis DNA Hydrogen-Ion Concentration Blotting Northern Immunohistochemistry Sperm Cell biology Fertility Polyclonal antibodies Sperm Motility biology.protein Calcium Female Rabbits Sperm Capacitation |
| Zdroj: | Frontiers in Bioscience. :566-581 |
| ISSN: | 1945-0508 1945-0494 |
| Popis: | Sodium-hydrogen exchanger as a channel for regulation of intracellular pH might be a crucial modulator of sperm capacitation and motility. Three members of this family have been identified in spermatozoa. A novel protein testis-specific sodium-hydrogen exchanger named mtsNHE was cloned in the present study. The mtsNHE localizing on principle piece of sperm flagellum contained 12 predicted transmembrane regions without cytoplasmic fragment at carboxyl terminus. Hydrophilic region was common in the sodium-hydrogen exchanger family members. Polyclonal antibodies to trans-membrane region significantly reduced sperm motility, acrosome reaction and ratio of in vitro fertilization. By in-pouring the antibodies in sperm solution, intracellular pH and calcium concentration were decreased. Muscle injection of female mice with the specific gene vaccine of mtsNHE, significantly stepped down fertility rate. Considering its specific expression and involvement in the regulation of fertility, the mtsNHE might be a potential target molecule for developing a new male contraceptive. |
| Databáze: | OpenAIRE |
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