Apple SUMO E3 ligase MdSIZ1 is involved in the response to phosphate deficiency
| Autor: | Yuan-Yuan Li, Rui-Fen Zhang, Chun-Xiang You, Yu-Jin Hao, Guang-Li Sha, Li-Jie Zhou |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Physiology Transgene Arabidopsis SUMO protein Plant Science 01 natural sciences Phosphates Ligases 03 medical and health sciences Gene Expression Regulation Plant MYB Plant Proteins Expression vector biology fungi Wild type Sumoylation Biotic stress Plants Genetically Modified biology.organism_classification Cell biology 030104 developmental biology Malus Callus Agronomy and Crop Science 010606 plant biology & botany |
| Zdroj: | Journal of Plant Physiology. 232:216-225 |
| ISSN: | 0176-1617 |
| DOI: | 10.1016/j.jplph.2018.11.012 |
| Popis: | In plants, SIZ1 regulates abiotic and biotic stress responses by promoting the SUMOylation of proteins. The apple MdSIZ1 protein has conserved domains similar to those of Arabidopsis AtSIZ1. Real-time fluorescent quantitative analysis showed that MdSIZ1 gene expression was induced by phosphate-deficient conditions. In addition, the level of SUMOylation was also significantly increased under these conditions. The MYB transcription factor MdPHR1 might be a target for the SUMO protein, which is a phosphorus starvation-dependent protein. Subsequently, an MdSIZ1 expression vector was constructed and transformed in Arabidopsis mutant siz1-2 and apple callus. The MdSIZ1 transgenic Arabidopsis partially complemented the defect phenotype of siz1-2 under phosphate-deficient conditions. The survival rate, length of primary root, and number or density of lateral roots were similar between the transgenic lines and wild type (WT). Under phosphate-deficient conditions, the SUMO conjugate and fresh weight of the MdSIZ1 transgenic apple callus were improved compared with WT. The MdSIZ1 transgenic apple callus grew under phosphate-deficient conditions, whereas the MdSIZ1 sense apple callus did not. Therefore, MdSIZ1 is involved in the regulation of the phosphate-deficiency response in apple. |
| Databáze: | OpenAIRE |
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