Effect of metformin on the proliferation, apoptosis, invasion and autophagy of ovarian cancer cells
Autor: | Ge Zou, Dandan Li, Yan Chen, Jie Bai |
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Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
Cancer Research endocrine system diseases Cell SKOV3 Flow cytometry 03 medical and health sciences 0302 clinical medicine Immunology and Microbiology (miscellaneous) Downregulation and upregulation medicine MTT assay Oncogene medicine.diagnostic_test Chemistry nutritional and metabolic diseases General Medicine Articles Cell cycle female genital diseases and pregnancy complications Metformin metastasis-associated 1 030104 developmental biology medicine.anatomical_structure ovarian cancer Apoptosis 030220 oncology & carcinogenesis Cancer research medicine.drug |
Zdroj: | Experimental and Therapeutic Medicine |
ISSN: | 1792-0981 |
Popis: | The present study evaluated the effect of metformin on the SKOV3 ovarian cancer cell line and investigated the underlying mechanism. The inhibitory rate of SKOV3 cells was analyzed by MTT assay. SKOV3 cell apoptosis rate was quantitatively measured using flow cytometry. The effect of metformin on intracellular autophagosomes was observed using electron microscopy. The migration and invasion capabilities of SKOV3 cells were assessed by cell scratch test and Transwell assay. Results demonstrated that. the proliferation rate of SKOV3 cells was significantly inhibited in a time- and concentration-dependent manner following treatment with different concentrations of metformin for 24, 48 and 72 h. The number of migratory cells significantly decreased with increasing concentrations of metformin. The administration of metformin also promoted autophagy of ovarian cancer The expression level of microtubule associated protein 1 light chain 3-α protein was markedly upregulated. The mRNA expression level of metastasis-associated 1 (MTA1) was significantly downregulated following metformin treatment. In conclusion, metformin intervention suppressed SKOV3 proliferation and induced apoptosis in a concentration-dependent manner. Metformin also inhibited the invasion and migration of SKOV3 cells. It was hypothesized that the underlying mechanism of metformin's effect may involve MTA1 downregulation. |
Databáze: | OpenAIRE |
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