OXA-427, a new plasmid-borne carbapenem-hydrolysing class D β-lactamase in Enterobacteriaceae

Autor: Didier Tandé, Warda Bouchahrouf, Laurent Dortet, Annette Schuermans, T.-D. Huang, Veroniek Saegeman, Stéphanie Evrard, Xavier De Bolle, Rémy A. Bonnin, Youri Glupczynski, Pierre Bogaerts, Thomas Jové, Thierry Naas
Rok vydání: 2017
Předmět:
Male
0301 basic medicine
Carbapenem
Imipenem
medicine.medical_treatment
Drug Resistance
Bacterial/genetics
Ceftazidime
Aztreonam
chemistry.chemical_compound
Belgium
Drug Resistance
Multiple
Bacterial
polycyclic compounds
Pharmacology (medical)
Temocillin
Cloning
Molecular
Plasmids/genetics
Escherichia coli/drug effects
biology
Anti-Bacterial Agents/metabolism
Hydrolysis
Enterobacteriaceae Infections
High-Throughput Nucleotide Sequencing
Middle Aged
Anti-Bacterial Agents
Infectious Diseases
Multiple
Plasmids
medicine.drug
Microbiology (medical)
Enterobacteriaceae/enzymology
030106 microbiology
beta-Lactamases/genetics
Microbial Sensitivity Tests
beta-Lactamases
Enterobacteriaceae Infections/epidemiology
Microbiology
03 medical and health sciences
Bacterial Proteins
Enterobacteriaceae
Carbapenems/metabolism
Escherichia coli
Journal Article
medicine
Humans
Belgium/epidemiology
Pharmacology
Bacterial Proteins/genetics
Molecular
biochemical phenomena
metabolism
and nutrition
Acinetobacter
biology.organism_classification
Carbapenems
chemistry
Beta-lactamase
bacteria
Aeromonas media
Cloning
Zdroj: Journal of Antimicrobial Chemotherapy. 72:2469-2477
ISSN: 1460-2091
0305-7453
DOI: 10.1093/jac/dkx184
Popis: Objectives: To describe a novel plasmid-borne class D carbapenemase (CHDL) named OXA-427 identified in several Enterobacteriaceae clinical isolates from nine patients in one Belgian hospital. Methods: OXA-427-producing isolates were analysed by an electrochemical imipenem hydrolysis method (BYG Carba test), Carba NP test, conventional phenotypic assays and by molecular methods (PCR, whole sequencing of the OXA-427-encoding plasmid and cloning). The antimicrobial resistance profile of OXA-427 was analysed by expression of the cloned gene in Escherichia coli DH10B and J53. Results: Eleven OXA-427-producing Enterobacteriaceae isolates of various species were identified from clinical specimens of nine patients between March 2012 and June 2014. OXA-427 shares only 22%-29% amino acid identity with OXA-48-like enzymes and other acquired CHDL (e.g. OXA-23, -24/40 and -58 of Acinetobacter spp.). Conversely, it appeared closely related to the chromosomal class D β-lactamase of Aeromonas media, Aeromonas hydrophila and Aeromonas sobria (99%, 89% and 77% of identity, respectively). When expressed in E. coli, OXA-427 hydrolysed imipenem and conferred resistance to extended-spectrum cephalosporins (mostly ceftazidime), penicillins including temocillin, and reduced susceptibility to carbapenems. The blaOXA-427 gene was located in a 45 kb resistance island on a 177 kb IncA/C plasmid. Conclusions: OXA-427 is a novel CHDL most closely related to chromosomal class D β-lactamase of A. media WS. It confers resistance to penicillins, ceftazidime and aztreonam and in some instances to carbapenems. OXA- 427, which is not detectable by classical molecular tests, caused a protracted outbreak in one university hospital over a 2 year period.
Databáze: OpenAIRE
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