The fermentation of lactulose in rats inoculated withClostridium paraputrificum influences the activities of liver and intestinal xenobiotic-metabolising enzymes

Autor: Jean-Claude Meslin, Evelyne F. Lhoste, Lionelle Nugon-Baudon, Sandrine Lory, Claude Andrieux
Přispěvatelé: Unité de recherche d'Écologie et Physiologie du Système Digestif (UEPSD), Institut National de la Recherche Agronomique (INRA)
Rok vydání: 2001
Předmět:
Zdroj: Journal of the Science of Food and Agriculture
Journal of the Science of Food and Agriculture, Wiley, 2001, 81, pp.1397-1404. ⟨10.1002/jsfa.957⟩
ISSN: 1097-0010
0022-5142
DOI: 10.1002/jsfa.957
Popis: One of the most recent hypotheses concerning the protective effect of some dietary fibres against colon cancer implicates butyrate. Very few publications have addressed its possible influence on the phase II enzymes and on the bacterial enzymes involved in the enterohepatic recirculation of toxic compounds. We used an original model of monoxenic rats oriented towards the preferential production of butyrate. Fischer male germ-free rats were mono-associated with a strain of Clostridium paraputrificum. They were fed either a control diet or a lactulose-enriched diet for 6 weeks. In the caeco-colon the specific activities (SAs) of the microsomal enzymes (glutathione-S-transferase and UDP-glucuronosyl-transferase) were 1.7-fold those of the control rats. In the intestine and liver the SAs of the phase II enzymes were not altered, nor was the hepatic cytochrome P450. In the caecum of the lactulose-fed rats the SA of β-glucuronidase decreased by 27% and that of β-glucosidase doubled. The chronic consumption of lactulose doubled the total caecal short-chain fatty acids, and the production of butyrate was enhanced. Since the germ-free rats behaved differently when fed the same diets, we concluded that the fermentation products of lactulose (and not the lactulose itself) were involved in the effects we describe. The involvement of butyrate in this phenomenon is discussed. Un modele animal (rat) produisant du butyrate est utilise. Une activite protectrice du butyrate sur le cancer du colon est recherche. les rats sont associes a une souche de Clostridium paraputrificum. Leur alimentation est enrichie en lactulose. La consommation de lactulose provoque un doublement des acides gras caecaux et l'augmentation de la synthese de butyrate. C'est le produit de fermentation du lactulose qui provoque les effets observes: augmentation des activites specifiques des enzymes microsomales (glutathione-S-transferase and UDP-glucuronosyl-transferase), les activites des enzymes de phase II et des enzymes a P450 ne sont pas modifiees.
Databáze: OpenAIRE
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