Biosynthesis and characterization of gold nanoparticles using Brazilian red propolis and evaluation of its antimicrobial and anticancer activities

Autor: Sérgio Ricardo Ambrósio, Rodrigo Cassio Sola Veneziani, Thayná de Souza Silva, Gari V. Ccana-Ccapatinta, Jairo Kenupp Bastos, Priscyla D. Marcato, Letícia B Silva, Caroline E. A. Botteon
Rok vydání: 2020
Předmět:
0301 basic medicine
Antioxidant
medicine.medical_treatment
Metal Nanoparticles
Diseases
02 engineering and technology
chemistry.chemical_compound
Anti-Infective Agents
Spectroscopy
Fourier Transform Infrared
Surface plasmon resonance
Cytotoxicity
Cancer
Multidisciplinary
Drug discovery
021001 nanoscience & nanotechnology
Antimicrobial
Chemistry
Biochemistry
Colloidal gold
Thermogravimetry
Medicine
0210 nano-technology
Cell biology
Science
Antineoplastic Agents
Microbial Sensitivity Tests
Microbiology
Propolis
Article
03 medical and health sciences
Biosynthesis
Microscopy
Electron
Transmission
Nanoscience and technology
Cell Line
Tumor
medicine
Humans
Bacteria
Fungi
Green Chemistry Technology
Surface Plasmon Resonance
In vitro
Materials science
030104 developmental biology
chemistry
Gold
Drug Screening Assays
Antitumor
Plant sciences
Zdroj: Scientific Reports
Scientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
ISSN: 2045-2322
Popis: Gold nanoparticles (AuNPs) are highlighted due to their low toxicity, compatibility with the human body, high surface area to volume ratio, and surfaces that can be easily modified with ligands. Biosynthesis of AuNPs using plant extract is considered a simple, low-cost, and eco-friendly approach. Brazilian Red Propolis (BRP), a product of bees, exhibits anti-inflammatory, anti-tumor, antioxidant, and antimicrobial activities. Here, we described the biosynthesis of AuNPs using BRP extract (AuNPextract) and its fractions (AuNPhexane, AuNPdichloromethane, AuNPethyl acetate) and evaluated their structural properties and their potential against microorganisms and cancer cells. AuNPs showed a surface plasmon resonance (SPR) band at 535 nm. The sizes and morphologies were influenced by the BRP sample used in the reaction. FTIR and TGA revealed the involvement of bioactive compounds from BRP extract or its fractions in the synthesis and stabilization of AuNPs. AuNPdichloromethane and AuNPhexane exhibited antimicrobial activities against all strains tested, showing their efficacy as antimicrobial agents to treat infectious diseases. AuNPs showed dose-dependent cytotoxic activity both in T24 and PC-3 cells. AuNPdichloromethane and AuNPextract exhibited the highest in vitro cytotoxic effect. Also, the cytotoxicity of biogenic nanoparticles was induced by mechanisms associated with apoptosis. The results highlight a potential low-cost green method using Brazilian red propolis to synthesize AuNPs, which demonstrated significant biological properties.
Databáze: OpenAIRE
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