Rickettsia conorii entry into Vero cells
| Autor: | Didier Raoult, N Teysseire, J A Boudier |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Time Factors
Lysis Phagocytosis Immunology Microbiology Rickettsiaceae Cytosol Phagosomes Chlorocebus aethiops Animals Rickettsia Vero Cells Phagosome biology bacterial infections and mycoses biology.organism_classification Virology Cell Compartmentation Microscopy Electron Infectious Diseases Vero cell Parasitology Rickettsia conorii Research Article |
| Zdroj: | Infection and Immunity. 63:366-374 |
| ISSN: | 1098-5522 0019-9567 |
| DOI: | 10.1128/iai.63.1.366-374.1995 |
| Popis: | The entry of rickettsiae into eukaryotic cells is mediated by an induced phagocytosis, but rickettsiae have never been observed in a closed phagocytic vacuole. In this study, Rickettsia conorii entry into Vero cells was observed by transmission electron microscopy during a period of 3 to 20 min after bacterium-cell contact. The entry occurred within 3 min after bacterium-cell contact, and R. conorii was observed in the process of engulfment, within a phagocytic vacuole, or free in the cytosol. Escape from the phagosome is a very rapid step since phagosome lysis was only occasionally observed. By 12 min, 90% of bacteria were internalized and half were free in the cytosol. This report confirms that rickettsiae penetrate nonphagocytic cells by induced phagocytosis and is the first demonstration of rickettsiae within a complete phagocytic vacuole. |
| Databáze: | OpenAIRE |
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