Significant improvement of heart function by cotransplantation of human mesenchymal stem cells and fetal cardiomyocytes in postinfarcted pigs
| Autor: | Jiang-Yong Min, Yinke Yang, Jian-Ping Zhang, Kimber L. Converso, Matthew F. Sullivan, Yong-F.u Xiao, James P. Morgan |
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| Rok vydání: | 2002 |
| Předmět: |
Male
Pulmonary and Respiratory Medicine Cardiac function curve endocrine system medicine.medical_specialty Swine Angiogenesis Heart Ventricles Myocardial Infarction Ventricular Function Left Mesoderm Andrology medicine Animals Humans Regeneration Myocyte Myocardial infarction Cells Cultured business.industry Multipotent Stem Cells Myocardium Regeneration (biology) Mesenchymal stem cell Hemodynamics equipment and supplies medicine.disease Myocardial Contraction Surgery Transplantation Microscopy Fluorescence cardiovascular system Stem cell Cardiology and Cardiovascular Medicine business |
| Zdroj: | The Annals of Thoracic Surgery. 74:1568-1575 |
| ISSN: | 0003-4975 |
| Popis: | Background Viable cardiomyocytes after myocardial infarction (MI) are unable to repair the necrotic myocardium due to their limited capability of regeneration. The present study investigated whether intramyocardial transplantation of human mesenchymal stem cells (hMSCs) or cotransplantation of hMSCs plus human fetal cardiomyocytes (hFCs; 1:1) reconstituted impaired myocardium and improved cardiac function in MI pigs. Methods and results Cultured hMSCs were transfected with green fluorescent protein (GFP). Six weeks after MI induction and cell transplantation, cardiac function was significantly improved in MI pigs transplanted with hMSCs alone. However, the improvement was even markedly greater in MI pigs cotransplanted with hMSCs plus hFCs. Histological examination demonstrated that transplantation of hMSCs alone or hMSCs plus hFCs formed GFP-positive engrafts in infarcted myocardium. In addition, immunostaining for cardiac α-myosin heavy chain and troponin I showed positive stains in infarcted regions transplanted with hMSCs alone or hMSCs plus hFCs. Conclusions Our data demonstrate that transplantation of hMSCs alone improved cardiac function in MI pigs with a markedly greater improvement from cotransplantation of hMSCs plus hFCs. This improvement might result from myocardial regeneration and angiogenesis in injured hearts by engrafted cells. |
| Databáze: | OpenAIRE |
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