Molecular electron paramagnetic resonance imaging of melanin in melanomas: a proof-of-concept
Autor: | Bernard Gallez, Jean-François Baurain, Olivier Feron, Mustapha Dinguizli, Julie DeWever, Emilia Sabina Vanea, Nicolas Charlier |
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Rok vydání: | 2008 |
Předmět: |
Chemistry
Electron paramagnetic resonance imaging Radical Melanoma Electron Spin Resonance Spectroscopy Early detection High resolution medicine.disease law.invention Melanin Mice Inbred C57BL Mice Nuclear magnetic resonance In vivo law medicine Molecular Medicine Animals Humans Radiology Nuclear Medicine and imaging Electron paramagnetic resonance Spectroscopy |
Zdroj: | NMR in biomedicine. 21(3) |
ISSN: | 0952-3480 |
Popis: | The incidence of malignant melanoma is increasing at an alarming rate. As the clinical outcome of the disease strongly depends on the localization of the lesion, early detection at the initial stages of development is critical. Here, we suggest spatial characterization of melanoma based on the presence of endogenous stable free radicals in melanin pigments. Taking into account the abundance of these naturally occurring free radicals in proliferating melanocytes and their localization pattern, we hypothesized that electron paramagnetic resonance (EPR) imaging could be a unique tool for mapping melanomas with high sensitivity and high resolution. The potential of EPR to image melanoma samples was demonstrated in vitro in animal and human samples. Using EPR systems operating at low frequency, we were also able to record in vivo EPR spectra and images from the melanin present in a subcutaneous melanoma implanted in a mouse. In addition to the proof-of-concept and the achievement of providing the first non-invasive image of an endogenous radical, this technology may represent a key advance in improving the diagnosis of suspected melanoma lesions. Copyright (c) 2008 John Wiley & Sons, Ltd. |
Databáze: | OpenAIRE |
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