Gene Expression Changes With Time in Skeletal Muscle of Severely Burned Children
| Autor: | Robert E. Barrow, Mohan R. Dasu, David N. Herndon |
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| Rok vydání: | 2005 |
| Předmět: |
Male
Pathology medicine.medical_specialty Adolescent Body Surface Area Vastus lateralis muscle Blotting Western Burn Units Muscle Proteins Physiology Sampling Studies Injury Severity Score Reference Values Biopsy medicine Humans RNA Messenger Child Muscle Skeletal Oligonucleotide Array Sequence Analysis Probability Body surface area Wound Healing medicine.diagnostic_test business.industry Beta-2 microglobulin Microarray analysis techniques Gene Expression Profiling Snap Skeletal muscle Original Articles Skin Transplantation Plastic Surgery Procedures medicine.anatomical_structure Case-Control Studies Child Preschool Hypermetabolism Female Surgery Burns business Follow-Up Studies |
| Zdroj: | Annals of Surgery. 241:647-653 |
| ISSN: | 0003-4932 |
| DOI: | 10.1097/01.sla.0000157266.53903.41 |
| Popis: | OBJECTIVE The purpose of this study was to identify gene-expression changes in leg muscle for up to 24 months after a severe thermal injury. SUMMARY BACKGROUND DATA Hypermetabolism associated with severe burns was thought to cease with wound healing and closure. It has been recently shown that hypermetabolism does not completely resolve after healing, and muscle catabolism continues after hospital discharge; however, just how long after discharge has not been established. METHODS : Six children, admitted to our hospital within 1 week after injury, were studied. Patients ranged in age from 3 to 18 years, with flame or scald burns covering more than 40% of their body surface area. At 1.5, 6, 12, 18, and 24 months postburn, a biopsy of the vastus lateralis muscle was taken and snap frozen at -80 degrees C. Total RNA was isolated and in vitro transcribed and hybridized to HG-U95 Av.2 Affymetrix arrays. The images were scanned and analyzed using Affymetrix GeneChip Analysis Suite 5.2 and dChip programs. Using 1 to 7 days after injury as baseline, comparisons were made of expression profiles at the various time intervals after injury. RESULTS When comparisons are made to nonburned children, 38 genes were significantly altered at 1.5 months, 10 genes remained altered at 6 months, 4 remained altered at 12 months, and 2 at 18 months. No differences could be shown at 24 months. Western blot analysis of beta-2 microglobulin and myosin light chain was used to corroborate the microarray data. CONCLUSIONS Gene changes can be identified for up to 18 months after burn but not at 24 months. These gene changes may provide information concerning what genes in skeletal muscle contribute to recovery from burn trauma. |
| Databáze: | OpenAIRE |
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