Expression of Nestin, Vimentin, and NCAM by Renal Interstitial Cells after Ischemic Tubular Injury
| Autor: | Annabel Gossiaux, David Vansthertem, Denis Nonclercq, Alexandre Legrand, Anne-Emilie Decleves, Nathalie Caron, Gérard Toubeau |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Male
Pathology medicine.medical_specialty Article Subject Health Toxicology and Mutagenesis lcsh:Biotechnology Population lcsh:Medicine Nerve Tissue Proteins Vimentin Interstitial cell S Phase Kidney Tubules Proximal Nestin Intermediate Filament Proteins Ischemia Proliferating Cell Nuclear Antigen lcsh:TP248.13-248.65 Genetics medicine Animals Rats Wistar education Neural Cell Adhesion Molecules Molecular Biology Cell Proliferation education.field_of_study Kidney biology Lineage markers lcsh:R General Medicine Immunohistochemistry Actins Rats Proliferating cell nuclear antigen medicine.anatomical_structure Bromodeoxyuridine Microscopy Fluorescence biology.protein Molecular Medicine Neural cell adhesion molecule Research Article Biotechnology |
| Zdroj: | Journal of Biomedicine and Biotechnology, Vol 2010 (2010) Journal of Biomedicine and Biotechnology |
| ISSN: | 1110-7243 |
| DOI: | 10.1155/2010/193259 |
| Popis: | This work explores the distribution of various markers expressed by interstitial cells in rat kidneys after ischemic injury (35 minutes) during regeneration of S3 tubules of outer stripe of outer medulla (OSOM). Groups of experimental animals (n=4) were sacrificed every two hours during the first 24 hours post-ischemia as well as 2, 3, 7, 14 days post-ischemia. The occurrence of lineage markers was analyzed on kidney sections by immunohistochemistry and morphometry during the process of tubular regeneration. In postischemic kidneys, interstitial cell proliferation, assessed by 5-bromo-2′-deoxyuridine (BrdU) and Proliferating Cell Nuclear Antigen (PCNA) labeling, was prominent in outer medulla and reach a maximum between 24 and 72 hours after reperfusion. This population was characterized by the coexpression of vimentin and nestin. The density of -Neural Cell Adhesion Molecule (NCAM) positive interstitial cells increased transiently (18–72 hours) in the vicinity of altered tubules. We have also localized a small population ofα-Smooth Muscle Actin (SMA)-positive cells confined to chronically altered areas and characterized by a small proliferative index. In conclusion, we observed in the postischemic kidney a marked proliferation of interstitial cells that underwent transient phenotypical modifications. These interstitial cells could be implicated in processes leading to renal fibrosis. |
| Databáze: | OpenAIRE |
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