Mechanism of biomolecular recognition of trimethyllysine by the fluorinated aromatic cage of KDM5A PHD3 finger

Autor: Thijs Beuming, F. Matthias Bickelhaupt, Maud H. M. Wuts, Ger J. M. Pruijn, Jos J. A. G. Kamps, Jasmin Mecinović, Bas J. G. E. Pieters, Jordi Poater, Woody Sherman, Robert S. Paton, Kiran Kumar, Paul B. White
Přispěvatelé: Chemistry and Pharmaceutical Sciences, AIMMS
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Pieters, B J G E, Wuts, M H M, Poater, J, Kumar, K, White, P B, Kamps, J J A G, Sherman, W, Pruijn, G J M, Paton, R S, Beuming, T, Bickelhaupt, F M & Mecinović, J 2020, ' Mechanism of biomolecular recognition of trimethyllysine by the fluorinated aromatic cage of KDM5A PHD3 finger ', Communications Chemistry, vol. 3, no. 1, 69, pp. 1-12 . https://doi.org/10.1038/s42004-020-0313-2
Communications Chemistry, 3
Pieters, B J G E, Wuts, M H M, Poater, J, Kumar, K, White, P B, Kamps, J J A G, Sherman, W, Pruijn, G J M, Paton, R S, Beuming, T, Bickelhaupt, F M & Mecinović, J 2020, ' Mechanism of biomolecular recognition of trimethyllysine by the fluorinated aromatic cage of KDM5A PHD3 finger ', Communications Chemistry, vol. 3, 69 . https://doi.org/10.1038/s42004-020-0313-2
Communications Chemistry, 3(1):69, 1-12. Springer Nature
Communications Chemistry
Communications Chemistry, 3, 1
Communications Chemistry, Vol 3, Iss 1, Pp 1-12 (2020)
ISSN: 2399-3669
DOI: 10.1038/s42004-020-0313-2
Popis: The understanding of biomolecular recognition of posttranslationally modified histone proteins is centrally important to the histone code hypothesis. Despite extensive binding and structural studies on the readout of histones, the molecular language by which posttranslational modifications on histone proteins are read remains poorly understood. Here we report physical-organic chemistry studies on the recognition of the positively charged trimethyllysine by the electron-rich aromatic cage containing PHD3 finger of KDM5A. The aromatic character of two tryptophan residues that solely constitute the aromatic cage of KDM5A was fine-tuned by the incorporation of fluorine substituents. Our thermodynamic analyses reveal that the wild-type and fluorinated KDM5A PHD3 fingers associate equally well with trimethyllysine. This work demonstrates that the biomolecular recognition of trimethyllysine by fluorinated aromatic cages is associated with weaker cation–π interactions that are compensated by the energetically more favourable trimethyllysine-mediated release of high-energy water molecules that occupy the aromatic cage. Lysine trimethylation is a key post-translational modification, which some epigenetic reader proteins detect through binding to aromatic cages involving cation-π interactions. Here systematic modification of the aromatic cage reveals that weaker cation-π interactions do not correlate with weaker binding owing to a compensating release of bound water molecules.
Databáze: OpenAIRE
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