Second- and third-generation biodiesel production with immobilised recombinant Rhizopus oryzae lipase : Influence of the support, substrate acidity and bioprocess scale-up
| Autor: | Francisco Valero, Maria Dolors Benaiges, Josu López-Fernández |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
Environmental Engineering Rhizopus oryzae Jatropha Bioengineering 010501 environmental sciences 01 natural sciences Catalysis 010608 biotechnology Substrate acidity Organic chemistry Immobilisation Lipase Waste Management and Disposal 0105 earth and related environmental sciences Biodiesel biology Renewable Energy Sustainability and the Environment Chemistry Rhizopus oryzae lipase Substrate (chemistry) General Medicine Transesterification biology.organism_classification Biodiesel production biology.protein Biocatalysis |
| Zdroj: | Universitat Autònoma de Barcelona Dipòsit Digital de Documents de la UAB |
| Popis: | Altres ajuts: Beca del Gobierno Vasco para la formación de investigadores predoctorales PRE_2017_1_0110 Altres ajuts: acords transformatius de la UAB Rhizopus oryzae lipase immobilised onto differently functionalised polymethacrylate (Purolite®) and magnetite superparamagnetic supports was assessed as a catalyst for biodiesel production with pomace oil. The presence of surface hydrocarbon chains increased the operational stability of the biocatalysts supported on Purolite® and superparamagnetic particles up to 9 and 2 times, respectively. By contrast, the presence of functional groups had no effect on the initial transesterification rate, which was twice higher with the lipase immobilised onto Purolite®. Also, functionalising Purolite® with epoxide and octadecyl groups led to the highest biodiesel and volumetric productivity. This biocatalyst with other substrates including makauba, jatropha, waste cooking oil, and microbial oil, led to similar initial reaction rates. However, simply raising substrate acidity from 0.5 to 2% increased the operational stability of the biocatalysts 15 times. A synergistic effect between acyl-acceptor concentration and substrate acidity was observed. The transesterification reaction was successfully scaled up to 50 mL. |
| Databáze: | OpenAIRE |
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