Constitutive production of c-di-GMP is associated with mutations in a variant of Pseudomonas aeruginosa with altered membrane composition
| Autor: | Juliane Düvel, Andrea Blanka, Christiane Ritter, Franz Narberhaus, Wolf-Rainer Abraham, Volkhard Kaever, Susanne Häussler, Birgit Klinkert, Andreas Dötsch |
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| Přispěvatelé: | Institute for Molecular Bacteriology, TWINCORE,30625 Hannover, Germany. |
| Rok vydání: | 2015 |
| Předmět: |
Translational efficiency
Sodium Chloride medicine.disease_cause Biochemistry Microbiology 03 medical and health sciences Cytosol Bacterial Proteins Sequence Homology Nucleic Acid Gene cluster medicine RNA Messenger Molecular Biology Cyclic GMP 030304 developmental biology 0303 health sciences Mutation Messenger RNA biology Base Sequence 030306 microbiology Pseudomonas aeruginosa Point mutation Cell Membrane Fatty Acids Biofilm Cell Biology biology.organism_classification Phenotype Biofilms Multigene Family Protein Biosynthesis Nucleic Acid Conformation 5' Untranslated Regions Bacteria Acetyl-CoA Carboxylase Signal Transduction |
| Zdroj: | Science signaling. 8(372) |
| ISSN: | 1937-9145 |
| Popis: | Most bacteria can form multicellular communities called biofilms on biotic and abiotic surfaces. This multicellular response to surface contact correlates with an increased resistance to various adverse environmental conditions, including those encountered during infections of the human host and exposure to antimicrobial compounds. Biofilm formation occurs when freely swimming (planktonic) cells encounter a surface, which stimulates the chemosensory-like, surface-sensing system Wsp and leads to generation of the intracellular second messenger 3',5'-cyclic-di-guanosine monophosphate (c-di-GMP). We identified adaptive mutations in a clinical small colony variant (SCV) of Pseudomonas aeruginosa and correlated their presence with self-aggregating growth behavior and an enhanced capacity to form biofilms. We present evidence that a point mutation in the 5' untranslated region of the accBC gene cluster, which encodes components of an enzyme responsible for fatty acid biosynthesis, was responsible for a stabilized mRNA structure that resulted in reduced translational efficiency and an increase in the proportion of short-chain fatty acids in the plasma membrane. We propose a model in which these changes in P. aeruginosa serve as a signal for the Wsp system to constitutively produce increased amounts of c-di-GMP and thus play a role in the regulation of adhesion-stimulated bacterial responses. |
| Databáze: | OpenAIRE |
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