Investigating the Link between Molecular Subtypes of Glioblastoma, Epithelial-Mesenchymal Transition, and CD133 Cell Surface Protein
| Autor: | Sheila K. Singh, Sendurai A. Mani, Hadi Zarkoob, Mohammad Kohandel, Joseph H. Taube |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
lcsh:Medicine
0302 clinical medicine Molecular Cell Biology Basic Cancer Research Tumor Cells Cultured Genome Databases Cluster Analysis AC133 Antigen lcsh:Science Neurological Tumors Cells Cultured Oligonucleotide Array Sequence Analysis Regulation of gene expression 0303 health sciences Multidisciplinary medicine.diagnostic_test Brain Neoplasms Genomics Cadherins Flow Cytometry Neural stem cell Oncology 030220 oncology & carcinogenesis embryonic structures Medicine Cellular Types Research Article Epithelial-Mesenchymal Transition Biology Flow cytometry 03 medical and health sciences Antigens CD Genetics Cancer Genetics medicine Humans Epithelial–mesenchymal transition Gene Glycoproteins 030304 developmental biology Cadherin Gene Expression Profiling lcsh:R Cell Membrane Mesenchymal stem cell Computational Biology Cancers and Neoplasms Epithelial Cells Molecular biology Gene expression profiling Cancer research lcsh:Q Glioblastoma Peptides Glioblastoma Multiforme |
| Zdroj: | PLoS ONE PLoS ONE, Vol 8, Iss 5, p e64169 (2013) |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0064169 |
| Popis: | In this manuscript, we use genetic data to provide a three-faceted analysis on the links between molecular subclasses of glioblastoma, epithelial-to-mesenchymal transition (EMT) and CD133 cell surface protein. The contribution of this paper is three-fold: First, we use a newly identified signature for epithelial-to-mesenchymal transition in human mammary epithelial cells, and demonstrate that genes in this signature have significant overlap with genes differentially expressed in all known GBM subtypes. However, the overlap between genes up regulated in the mesenchymal subtype of GBM and in the EMT signature was more significant than other GBM subtypes. Second, we provide evidence that there is a negative correlation between the genetic signature of EMT and that of CD133 cell surface protein, a putative marker for neural stem cells. Third, we study the correlation between GBM molecular subtypes and the genetic signature of CD133 cell surface protein. We demonstrate that the mesenchymal and neural subtypes of GBM have the strongest correlations with the CD133 genetic signature. While the mesenchymal subtype of GBM displays similarity with the signatures of both EMT and CD133, it also exhibits some differences with each of these signatures that are partly due to the fact that the signatures of EMT and CD133 are inversely related to each other. Taken together these data shed light on the role of the mesenchymal transition and neural stem cells, and their mutual interaction, in molecular subtypes of glioblastoma multiforme. |
| Databáze: | OpenAIRE |
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