Colocalization of leptin receptor (OB-R) mRNA and placental lactogen-II in rat trophoblast cells: gestational profile of OB-R mRNA expression in placentae
| Autor: | Motoyuki Kawai, Shizuka Otani, Masaaki Yamaguchi, Takashi Murakami, Kurajiro Kishi, Kenji Shima |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
medicine.medical_specialty
Time Factors Biophysics Gene Expression Receptors Cell Surface In situ hybridization Biology Biochemistry Andrology Rats Sprague-Dawley Pregnancy Internal medicine medicine Animals Protein Isoforms Northern blot RNA Messenger Molecular Biology reproductive and urinary physiology Cellular localization In Situ Hybridization Leptin receptor Decidua Colocalization Trophoblast Cell Biology medicine.disease Blotting Northern Placental Lactogen Immunohistochemistry Rats Trophoblasts Alternative Splicing medicine.anatomical_structure Endocrinology embryonic structures Receptors Leptin Female Carrier Proteins |
| Zdroj: | Biochemical and biophysical research communications. 257(2) |
| ISSN: | 0006-291X |
| Popis: | The present study was designed to clarify the cellular localization and expression of leptin receptor(s) [OB-R(s)] mRNA including its splice variants and their correlation with the cells which secrete placental hormone, placental lactogen-II (PL-II), in rat placentae. By in situ hybridization analysis, hybridization signals for OB-Rb and the common extracellular domain of OB-R were first detectable in some cells of the labyrinth zone of the placentae on day 14 of pregnancy and then a lot of cells dispersed in the entire area of the labyrinth zone expressed OB-Rb during the latter half of pregnancy. However, no expression was observed in the decidua and the junctional zone of the placentae during pregnancy. Double staining study revealed that signals for OB-R expressing trophoblast cells showed PL-II immunoreactivity in the labyrinth zone of the placentae. In Northern blot analysis, two bands (2.8 kb and 5.1 kb) of OB-R mRNA expression were observed in the placentae from day 17 to 21 of pregnancy and the expression of both increased markedly up to day 21 of pregnancy. RT-PCR analysis revealed that OB-Rb, OB-Ra, and OB-Re are expressed in the placentae on days 19 and 21 of pregnancy. These results suggest that the OB-R may have a physiological significance in the placental function during the latter half of pregnancy. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect