Effect of graphene oxide on affinity-immobilization of purple membranes on solid supports
| Autor: | Kai-Ru Jheng, Chi-Jung Lin, Aline Natasia Kosasih, Hsiu-Mei Chen, Jia-Yaw Chang |
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| Rok vydání: | 2013 |
| Předmět: |
Halobacterium salinarum
Materials science Fabrication Oxidized Avidin Surface Properties Oxide Analytical chemistry law.invention chemistry.chemical_compound Colloid and Surface Chemistry Adsorption Purple Membrane law Surface roughness Physical and Theoretical Chemistry Molecular Structure Graphene Oxides Surfaces and Interfaces General Medicine chemistry Chemical engineering Biotinylation Bacteriorhodopsins Graphite Layer (electronics) Biotechnology |
| Zdroj: | Colloids and surfaces. B, Biointerfaces. 116 |
| ISSN: | 1873-4367 |
| Popis: | The effect of graphene oxide (GO) on the surface fabrication of purple membranes (PM) containing photosensitive bacteriorhodopsin is first reported in this study. GO was initially modified with biotin and then coupled with oxidized avidin to generate a GO–avidin complex, which was subsequently used as a linker to immobilize biotinylated PM (b-PM) onto amine-functionalized supports. Indium–tin–oxide glass coated with the GO–avidin complex was more hydrophilic than the electrode coated only with oxidized avidin, and the successive b-PM adsorption yielded a 1.4-fold higher (410 nA/cm 2 ) photoelectric activity. AFM analysis on mica revealed that the GO–avidin complex layer had less surface roughness and dissipation energy than the pure oxidized avidin linker layer. For subsequent b-PM fabrication, GO addition not only reduced the stacking of immobilized b-PM patches but also improved their interior compactness and surface smoothness. This study demonstrates a convenient way to introduce GO into PM fabrication technology to provide enhanced surface morphology and photoelectric activity. |
| Databáze: | OpenAIRE |
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